{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:25:27Z","timestamp":1779902727088,"version":"3.53.1"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,3,7]],"date-time":"2013-03-07T00:00:00Z","timestamp":1362614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2013,10]]},"abstract":"Abstract<\/jats:title>BACKGROUND<\/jats:title>A key challenge for designing RNAi<\/jats:styled-content>\u2010based crop protection strategies is the identification of effective target genes in the pathogenic organism. In this study, in vitro<\/jats:italic> antifungal activities of a set of synthetic double\u2010stranded RNA<\/jats:styled-content> molecules on spore germination of two major pathogenic fungi of banana, Fusarium oxysporum<\/jats:italic> Schlecht f. sp. cubense<\/jats:italic> WC<\/jats:styled-content> Snyder & HN<\/jats:styled-content> Hans (Foc) and Mycosphaerella fijiensis<\/jats:italic> Morelet (Mf) were evaluated<\/jats:bold>.<\/jats:p><\/jats:sec>RESULTS<\/jats:title>All the tested synthetic dsRNAs<\/jats:styled-content> successfully triggered the silencing of target genes and displayed varying degrees of potential to inhibit spore germination of both tested banana pathogens. When Foc dsRNAs<\/jats:styled-content> were applied to Foc spores, inhibition ranged from 79.8 to 93.0%, and from 19.9 to 57.8% when Foc dsRNAs<\/jats:styled-content> were applied to Mf spores. However, when Mf dsRNAs<\/jats:styled-content> were applied on Mf spores, inhibition ranged from 34.4 to 72.3%, and from 89.7 to 95.9% when Mf dsRNAs<\/jats:styled-content> were applied to Foc spores<\/jats:bold>.<\/jats:p><\/jats:sec>CONCLUSION<\/jats:title>The dsRNAs<\/jats:styled-content> for adenylate cyclase, DNA<\/jats:styled-content> polymerase alpha subunit and DNA<\/jats:styled-content> polymerase delta subunit showed high levels of spore germination inhibition during both self\u2010 and cross\u2010species tests, making them the most promising targets for RNA<\/jats:styled-content>\u2010mediated resistance in banana against these fungal pathogens. \u00a9 2013 Society of Chemical Industry<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.3480","type":"journal-article","created":{"date-parts":[[2013,1,3]],"date-time":"2013-01-03T11:31:14Z","timestamp":1357212674000},"page":"1155-1162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["In vitro<\/i> antifungal activity of synthetic dsRNA<\/scp> molecules against two pathogens of banana, Fusarium oxysporum<\/i> f. sp. cubense<\/i> and Mycosphaerella fijiensis<\/i>"],"prefix":"10.1002","volume":"69","author":[{"given":"Francis M","family":"Mumbanza","sequence":"first","affiliation":[{"name":"National Banana Research Programme National Agriculture Research Organisation Kampala Uganda"},{"name":"Department of Crop Production Makerere University Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Kiggundu","sequence":"additional","affiliation":[{"name":"National Agricultural Research Laboratories National Agriculture Research Organisation Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Tusiime","sequence":"additional","affiliation":[{"name":"Department of Crop Production Makerere University Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilberforce K","family":"Tushemereirwe","sequence":"additional","affiliation":[{"name":"National Banana Research Programme National Agriculture Research Organisation Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuck","family":"Niblett","sequence":"additional","affiliation":[{"name":"Venganza, Inc. 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