{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T01:11:31Z","timestamp":1773882691800,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T00:00:00Z","timestamp":1369353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:bold>Hybrid corn varieties exhibit benefits associated with heterosis and account for most of the corn acreage in the <jats:styled-content style=\"fixed-case\">USA<\/jats:styled-content>. Hybrid seed corn is produced by crossing a female parent which is male\u2010sterile and therefore incapable of self\u2010pollination with a male parent as the pollen donor. The majority of hybrid seed corn is produced by mechanical detasseling which involves physically removing the tassel, a process that is laborious and costly.<\/jats:bold><\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:bold>Glyphosate\u2010resistant corn was developed via expression of a glyphosate insensitive 5\u2010enolpyruvyl\u2010shikimate 3\u2010phosphate synthase enzyme (<jats:styled-content style=\"fixed-case\">CP4\u2010EPSPS<\/jats:styled-content>). Experimentation with molecular expression elements resulted in selective reduction of <jats:styled-content style=\"fixed-case\">CP4\u2010EPSPS<\/jats:styled-content> expression in male reproductive tissues. The resulting plant demonstrated sterile tassel following glyphosate application with little to no injury to the rest of the plant. Using <jats:styled-content style=\"fixed-case\"><jats:sup>14<\/jats:sup>C<\/jats:styled-content>\u2010glyphosate as a marker, we also examined the translocation of glyphosate to the tassel via spray application in a track sprayer to simulate field application. The results allowed optimization of spray parameters such as dose, spray timing and target to maximize tassel delivery of glyphosate for efficient sterilization.<\/jats:bold><\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:bold>The Roundup hybridization system (<jats:styled-content style=\"fixed-case\">RHS<\/jats:styled-content>) is a novel process for hybrid seed production based on glyphosate\u2010mediated male sterility. <jats:styled-content style=\"fixed-case\">RHS<\/jats:styled-content> replaces mechanical detasseling with glyphosate spray and greatly simplifies the process of hybrid seed corn production. \u00a9 2013 Society of Chemical Industry<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.3526","type":"journal-article","created":{"date-parts":[[2013,3,4]],"date-time":"2013-03-04T23:48:53Z","timestamp":1362440933000},"page":"212-218","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Improving hybrid seed production in corn with\u00a0glyphosate\u2010mediated male sterility"],"prefix":"10.1002","volume":"70","author":[{"given":"Paul C C","family":"Feng","sequence":"first","affiliation":[{"name":"Monsanto Co.  800 N. 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