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The viral coat protein (<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>) has been assumed to play important roles in the entry of <jats:styled-content style=\"fixed-case\">TYLCV<\/jats:styled-content> into the insect midgut cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Testing the hypothesis that <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> plays an important role in <jats:styled-content style=\"fixed-case\">TYLCV<\/jats:styled-content> acquisition by <jats:italic>B. tabaci<\/jats:italic>, a soluble form of the <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> was expressed and purified. The purified recombinant <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> made it possible to examine the function of <jats:styled-content style=\"fixed-case\">TYLCV CP<\/jats:styled-content> without other viral proteins. In an <jats:italic>in vivo<\/jats:italic> binding assay, specific binding of <jats:styled-content style=\"fixed-case\">TYLCV CP<\/jats:styled-content> to <jats:italic>B. tabaci<\/jats:italic> midguts was detected when purified <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> was fed to <jats:italic>B. tabaci<\/jats:italic>. In addition, real\u2010time polymerase chain reaction analysis of virus titre revealed that <jats:italic>B. tabaci<\/jats:italic> fed with purified <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> had reduced the level of virus in their midgut compared with those fed with bovine serum albumin or maltose\u2010binding protein. These results suggest that binding of <jats:styled-content style=\"fixed-case\">TYLCV CP<\/jats:styled-content> to the <jats:italic>B. tabaci<\/jats:italic> midgut specifically inhibits virus acquisition.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The findings that <jats:styled-content style=\"fixed-case\">TYLCV CP<\/jats:styled-content> binds to <jats:italic>B. tabaci<\/jats:italic> midguts and decreases virus acquisition provide direct evidence that <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> mediates the attachment of <jats:styled-content style=\"fixed-case\">TYLCV<\/jats:styled-content> to receptors on the epithelial cells of the <jats:italic>B. tabaci<\/jats:italic> midgut. \u00a9 2014 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.3750","type":"journal-article","created":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T09:12:16Z","timestamp":1391159536000},"page":"1624-1631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Expression and functional characterisation of a soluble form of <i>Tomato yellow leaf curl virus<\/i> coat protein"],"prefix":"10.1002","volume":"70","author":[{"given":"Lan\u2010Lan","family":"Wang","sequence":"first","affiliation":[{"name":"Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue\u2010Mei","family":"Wei","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University Hangzhou 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