{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:29:16Z","timestamp":1761092956829,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,3,20]],"date-time":"2014-03-20T00:00:00Z","timestamp":1395273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Soon after the introduction of Qo inhibitor fungicides in 1996, the point mutation leading to the amino acid exchange glycine to alanine at the 143 position of the mitochondrial cytochrome <jats:italic>b<\/jats:italic> gene was identified as the main cause of resistance. The present study describes the role of anastomosis in the transmission of the <jats:styled-content style=\"fixed-case\">G143A<\/jats:styled-content> mutation in <jats:italic>Magnaporthe grisea<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Two <jats:italic>M. grisea<\/jats:italic> mutants were co\u2010cultivated on oatmeal agar and also co\u2010inoculated on barley leaves. The mutants differed by the presence of the <jats:styled-content style=\"fixed-case\">G143A<\/jats:styled-content> mutation in one isolate and a disrupted <jats:italic><jats:styled-content style=\"fixed-case\">AOX<\/jats:styled-content><\/jats:italic> gene by insertion of a hygromycin gene in the other (M\u2010145). Specific resistant (r) or sensitive (s) phenotypes of 409 monosporic cultures were determined on media amended with either hygromycin (H) or azoxystrobin (S) plus <jats:styled-content style=\"fixed-case\">SHAM<\/jats:styled-content>. The phenotypes identified reflected not only the phenotypes of mutants M\u2010145 and <jats:styled-content style=\"fixed-case\">G143A<\/jats:styled-content> but also the wild\u2010type parent phenotype <jats:styled-content style=\"fixed-case\">HsSs<\/jats:styled-content> and a new <jats:styled-content style=\"fixed-case\">HrSr<\/jats:styled-content> isolate.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Identification of the <jats:italic>M. grisea<\/jats:italic> phenotypes <jats:styled-content style=\"fixed-case\">HrSr<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HsSs<\/jats:styled-content> suggests that anastomosis occurred during co\u2010cultivation and co\u2010inoculation of the mutants M\u2010145 and <jats:styled-content style=\"fixed-case\">G143A<\/jats:styled-content>, allowing the transfer of the <jats:styled-content style=\"fixed-case\">G143A<\/jats:styled-content> point mutation from the <jats:styled-content style=\"fixed-case\">QoI<\/jats:styled-content>\u2010resistant isolate to the susceptible isolate. \u00a9 2014 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.3758","type":"journal-article","created":{"date-parts":[[2014,2,6]],"date-time":"2014-02-06T09:41:55Z","timestamp":1391679715000},"page":"1918-1823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transmission of the <scp>G143A QoI<\/scp>\u2010resistance point mutation through anastomosis in <i>Magnaporthe grisea<\/i>"],"prefix":"10.1002","volume":"70","author":[{"given":"Cruz","family":"Avila\u2010Adame","sequence":"first","affiliation":[{"name":"Discovery Research Dow AgroSciences LLC Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,20]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"239","volume-title":"Populations of Plant Pathogens: their Dynamics and Genetics","author":"Georgopoulos SG","year":"1987"},{"key":"e_1_2_5_3_1","first-page":"340","volume-title":"Plant Disease Epidemiology: Genetics, Resistance and Management","author":"Milgroom MG","year":"1989"},{"key":"e_1_2_5_4_1","first-page":"23","volume-title":"Modern Selective Fungicides","author":"Dekker J","year":"1995"},{"unstructured":"Crop Life. 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