{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T05:00:39Z","timestamp":1784782839863,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,3,20]],"date-time":"2014-03-20T00:00:00Z","timestamp":1395273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the High Technology Research and Development Program (863) of China","award":["2011AA10A204"],"award-info":[{"award-number":["2011AA10A204"]}]},{"name":"the Natural Science Foundation Project of CQ CSTC","award":["2010BB1231"],"award-info":[{"award-number":["2010BB1231"]}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2015,1]]},"abstract":"Abstract<\/jats:title>BACKGROUND<\/jats:title>Entomopathogenic fungi have been developed as biopesticides, but poor efficacy has blocked their application. One approach to improving virulence is by genetic manipulation. Bj\u03b1<\/jats:italic>IT<\/jats:styled-content> from the venom of Buthotus judaicus<\/jats:italic> is an insect\u2010selective neurotoxin. To clarify the insecticidal potency of Bj\u03b1<\/jats:italic>IT<\/jats:styled-content> as a virulence candidate in microbial biocontrol agents, the entomopathogenic fungus Metarhizium acridum<\/jats:italic> was genetically modified with Bj\u03b1<\/jats:italic>IT<\/jats:styled-content>, and its resulting activity against locusts (Locusta migratoria manilensis<\/jats:italic>) was assessed.<\/jats:p><\/jats:sec>RESULT<\/jats:title>In comparison with the wild\u2010type strain, the engineered isolate Bj\u03b1<\/jats:italic>IT<\/jats:styled-content>\u2010102 grew significantly quicker in locust haemolymph. Correspondingly, the median lethal dose (LC50<\/jats:sub><\/jats:styled-content>) for Bj\u03b1<\/jats:italic>IT<\/jats:styled-content>\u2010102 was 18.2\u2010fold lower, and the median lethal times (LT50<\/jats:sub><\/jats:styled-content>) for Bj\u03b1<\/jats:italic>IT<\/jats:styled-content>\u2010102 were reduced by 28.1 and 30.4%, respectively, after topical inoculation and injection. Bj\u03b1<\/jats:italic>IT<\/jats:styled-content>\u2010102 formed conidia on dead locusts, although the conidial yield was reduced 1.58\u2010fold. Moreover, there were no significant differences in germination and appressorium formation between the Bj\u03b1<\/jats:italic>IT<\/jats:styled-content>\u2010102 and wild\u2010type strains.<\/jats:p><\/jats:sec>CONCLUSION<\/jats:title>Expression of Bj\u03b1<\/jats:italic>IT<\/jats:styled-content> in M. acridum<\/jats:italic> significantly increased virulence against locusts by shortening the in vivo<\/jats:italic> infection period without affecting conidium formation on the carcasses. This study demonstrated that engineering entomopathogenic fungi to incorporate Bj\u03b1<\/jats:italic>IT<\/jats:styled-content> offers great potential for increasing their virulence. \u00a9 2014 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.3762","type":"journal-article","created":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T06:48:25Z","timestamp":1392706105000},"page":"58-64","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Integration of an insecticidal scorpion toxin (Bj\u03b1<\/i>IT<\/scp>) gene into Metarhizium acridum<\/i> enhances fungal virulence towards Locusta migratoria manilensis<\/i>"],"prefix":"10.1002","volume":"71","author":[{"given":"Guoxiong","family":"Peng","sequence":"first","affiliation":[{"name":"Genetic Engineering Research Center, School of Life Sciences Chongqing University Chongqing 400030 China"},{"name":"Chongqing Engineering Research Center for Fungal Insecticides Chongqing 400030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxian","family":"Xia","sequence":"additional","affiliation":[{"name":"Genetic Engineering Research Center, School of Life Sciences Chongqing University Chongqing 400030 China"},{"name":"Chongqing Engineering Research Center for Fungal Insecticides Chongqing 400030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(04)54001-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.2780270210"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2007.08.001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.46.1.667"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3852\/07-202"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0953756299001756"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2008.03.007"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.13.6349"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bcon.2001.0938"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-2306-z"},{"key":"e_1_2_6_12_1","unstructured":"FedericiBA BonningBCand St Leger RJ Improvement of insect pathogens as insecticides through genetic engineering inPathoBiotechnology ed. by Hill C and Sleator R. 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