{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T21:09:21Z","timestamp":1771189761985,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T00:00:00Z","timestamp":1494892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"\u2018Cooperative Research Program for Agriculture Science & Technology Development\u2019","award":["PJ01245703"],"award-info":[{"award-number":["PJ01245703"]}]},{"name":"Rural Development Administration, Republic of Korea"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>There is growing demand for the development of alternative pest control agents that are effective as well as non\u2010toxic to human health and the environment. Plant protection products derived from plant extracts are an eco\u2010friendly alternative to synthetic pesticides. The aim of this study was to identify larvicidal compounds isolated from a natural source against <jats:italic>Plutella xylostella<\/jats:italic> L. In a larvicidal activity assay, several solvent fractions from the methanol extract of <jats:italic>Piper nigrum<\/jats:italic> L. fruit showed larvicidal effects against <jats:italic>P. xylostella<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Screening results indicated that chloroform extract was the most effective against <jats:italic>P. xylostella<\/jats:italic> larvae. Two compounds with insecticidal activity in the chloroform fraction were identified as piperolein B and piperchabamide D by spectroscopic analyses, including mass spectrometry and <jats:styled-content style=\"fixed-case\">NMR<\/jats:styled-content>, and by comparison to published data. At applications of 0.1 mg\u2009<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup> concentration, piperolein B and piperchabamide D, respectively, induced 96.7\u2009\u00b1\u20095.8% and 79.2\u2009\u00b1\u200916.6% mortality rates of <jats:italic>P. xylostella<\/jats:italic> larvae 4 days post\u2010application.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Our results demonstrate that piperolein B and piperchabamide D isolated from <jats:italic>P. nigrum<\/jats:italic> are the major constituents of the extract demonstrating insecticidal properties for the control of <jats:italic>P. xylostella<\/jats:italic> larvae. These plant\u2010derived compounds should become useful alternatives to synthetic chemicals after studying their insecticidal mechanisms. \u00a9 2017 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.4580","type":"journal-article","created":{"date-parts":[[2017,3,28]],"date-time":"2017-03-28T02:26:38Z","timestamp":1490667998000},"page":"1564-1567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Piperolein B and piperchabamide D isolated from black pepper (<i>Piper nigrum<\/i> L.) as larvicidal compounds against the diamondback moth (<i>Plutella xylostella<\/i>)"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3374-9109","authenticated-orcid":false,"given":"Ki Seon","family":"Hwang","sequence":"first","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences Chungnam National University  Daejeon Republic of 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