{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T13:07:58Z","timestamp":1761311278038,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,20]],"date-time":"2017-10-20T00:00:00Z","timestamp":1508457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Attract\u2010and\u2010kill strategies, in which a behavior\u2010modifying stimulus (e.g. a pheromone) is integrated with a pest control agent, have lately attracted increased interest for mosquito control. Previously, it was demonstrated that the polyurea microencapsulated synthetic oviposition pheromone 6\u2010acetoxy\u20105\u2010hexadecanolide (MSP) was sufficiently attractive to gravid females of <jats:styled-content style=\"fixed-case\"><jats:italic>Culex pipiens<\/jats:italic><\/jats:styled-content> L. biotype <jats:italic>molestus<\/jats:italic> F\u00f8rskal (Diptera: Culicidae) for a period of 40 days. Furthermore, it was shown that MSP could be effectively combined with the organophosphate temephos to achieve efficient mosquito control. In the present study, the effect of the combined application of MSP with commonly used larvicides on the oviposition response of <jats:italic>Cx. p<\/jats:italic>. biotype <jats:italic>molestus<\/jats:italic> females over time was investigated in two\u2010choice oviposition bioassays. As larvicides, the insect growth regulator diflubenzuron and the bacterial insecticide spinosad were evaluated at their lowest recommended label dose, whereas temephos was used as a control.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>When MSP was applied in combination with diflubenzuron, the attractancy of MSP to gravid females was in all cases negatively affected, as fewer egg rafts were laid in pots treated with MSP and diflubenzuron compared with MSP alone. Spinosad did not reduce, but rather increased, the attractive effect of the oviposition pheromone at the beginning of the bioassay (at 2 days of ageing) when co\u2010applied; however, the observed attractive effect was significantly reduced after 7 days of ageing and remained stable at the same level until the termination of the bioassay. Finally, the oviposition pattern of egg rafts laid on the pot with MSP and temephos was similar to that of egg rafts laid on the pot with MSP alone, showing that temephos did not significantly affect MSP activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The results of the present study highlight the effects of the combined application of MSP with spinosad and diflubenzuron on the oviposition of gravid <jats:italic>Cx. p<\/jats:italic>. biotype <jats:italic>molestus<\/jats:italic> females, which in most cases eliminated the attractive effect of MSP. \u00a9 2017 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.4719","type":"journal-article","created":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T11:46:44Z","timestamp":1504007204000},"page":"392-397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of the combined application of microencapsulated synthetic oviposition pheromone (MSP) with different larvicidal agents on the oviposition of <scp><i>Culex pipiens<\/i><\/scp> biotype <i>molestus<\/i>"],"prefix":"10.1002","volume":"74","author":[{"given":"Antonios","family":"Michaelakis","sequence":"first","affiliation":[{"name":"Department of Entomology and Agricultural Zoology Benaki Phytopathological Institute  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios P","family":"Papachristos","sequence":"additional","affiliation":[{"name":"Department of Entomology and Agricultural Zoology Benaki Phytopathological Institute  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christos I","family":"Rumbos","sequence":"additional","affiliation":[{"name":"Laboratory of Entomology and Agricultural Zoology, Department of Agriculture, Crop Production and Rural Environment University of Thessaly  Magnesia Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christos G","family":"Athanassiou","sequence":"additional","affiliation":[{"name":"Laboratory of Entomology and Agricultural Zoology, Department of Agriculture, Crop Production and Rural Environment University of Thessaly  Magnesia Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects7040065"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.34.010189.002153"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0085-56262009000100002"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-37.1.172"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.2987\/8756-971X(2006)22[490:TATTFA]2.0.CO;2"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1408"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1079\/9781845939861.0095","volume-title":"Biological and Environmental Control of Disease Vectors","author":"Logan JG","year":"2013"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1418"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0639-2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005043"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.12681\/eh.11611","article-title":"Preliminary studies of the attract\u2013and\u2013kill strategy on hatched larvae of Culex pipiens (Diptera: Culicidae)","volume":"17","author":"Kioulos E","year":"2007","journal-title":"Entomol Hell"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jspr.2011.01.004"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.424"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B0-44-451924-6\/00078-8"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.2987\/09-5936.1"},{"key":"e_1_2_5_17_1","first-page":"151","article-title":"Evaluation of the naturally\u2010derived insecticide spinosad against Culex pipiens L. 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