{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:52:11Z","timestamp":1790283131138,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T00:00:00Z","timestamp":1519171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Banque public d'investissement (Bpi)","award":["ISI n\u00b0I1301001W \u201cNEORAMUS"],"award-info":[{"award-number":["ISI n\u00b0I1301001W \u201cNEORAMUS"]}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Soon after difenacoum began to be used, resistance to this rodenticide was detected in rats in northeast Hampshire and northwest Berkshire in England. Resistance to difenacoum has been reported to be stronger in rats from Berkshire than in rats from Hampshire. Surprisingly, after the discovery of the vitamin K epoxide reductase complex subunit 1 (\n                      <jats:italic>Vkorc1<\/jats:italic>\n                      ) gene, rats from Berkshire and Hampshire were all shown to be homozygous for the L120Q mutation in\n                      <jats:italic>Vkorc1<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      This study aimed to evaluate the resistance of Berkshire rats to confirm their extreme resistance and determine mechanisms supporting this resistance. For this purpose, we created a quasicongenic rat F7 strain by using a Berkshire rat as a donor to introduce the L120Q mutation in\n                      <jats:italic>Vkorc1<\/jats:italic>\n                      into the genetic background of an anticoagulant\u2010susceptible recipient strain. The use of F7 rats enabled demonstration of (i) the level of resistance to difenacoum conferred by the L120Q mutation, (ii) co\u2010dominance of the L120 and Q120 alleles, (iii) the extreme resistance of Berkshire rats compared with Q120\/Q120 rats as a consequence of additional resistance mechanisms, and (iv) the involvement of cytochrome P 450 (CYP450) enzymes in this extreme resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This study demonstrated that elevated CYP450 oxidative metabolism leading to accelerated difenacoum detoxification is involved in the Berkshire phenotype. \u00a9 2017 Society of Chemical Industry<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.4797","type":"journal-article","created":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T11:04:08Z","timestamp":1511175848000},"page":"1328-1334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Elevated difenacoum metabolism is involved in the difenacoum\u2010resistant phenotype observed in Berkshire rats homozygous for the L120Q mutation in the vitamin K epoxide reductase complex subunit 1 (\n                    <i>Vkorc1<\/i>\n                    ) gene"],"prefix":"10.1002","volume":"74","author":[{"given":"Maylis","family":"Boitet","sequence":"first","affiliation":[{"name":"USC 1233 RS2GP, VetAgro Sup, INRA University of Lyon  Marcy l'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdessalem","family":"Hammed","sequence":"additional","affiliation":[{"name":"USC 1233 RS2GP, VetAgro Sup, INRA University of Lyon  Marcy l'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nolan","family":"Chatron","sequence":"additional","affiliation":[{"name":"USC 1233 RS2GP, VetAgro Sup, INRA University of Lyon  Marcy l'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Val\u00e9ry","family":"Debaux","sequence":"additional","affiliation":[{"name":"USC 1233 RS2GP, VetAgro Sup, INRA University of Lyon  Marcy l'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Benoit","sequence":"additional","affiliation":[{"name":"USC 1233 RS2GP, VetAgro Sup, INRA University of Lyon  Marcy l'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3371-0952","authenticated-orcid":false,"given":"Virginie","family":"Lattard","sequence":"additional","affiliation":[{"name":"USC 1233 RS2GP, VetAgro Sup, INRA University of Lyon  Marcy l'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400046957"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400046969"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(07)00011-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01419.x"},{"key":"e_1_2_7_6_1","first-page":"149","article-title":"Forty five years of anticoagulant rodenticides \u2010 past, present and future trends","volume":"15","author":"Hadler MR","year":"1992","journal-title":"Proc Vert Pest Conf"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/188517a0"},{"key":"e_1_2_7_8_1","first-page":"1668","article-title":"Mice and spreading despite such poisons as warfarin","volume":"3746","author":"Dodsworth E","year":"1961","journal-title":"Munic Eng Lond"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.176.4041.1343"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02214"},{"key":"e_1_2_7_11_1","first-page":"39","article-title":"Distribution of anticoagulant resistance in the brown rat in Belgium. Belg","volume":"142","author":"Baert K","year":"2012","journal-title":"J Zool"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1869"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3862"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.040360"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.2254"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3309"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1458"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/FPC.0b013e32832ee55b"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.08.010"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1897\/04-022R.1"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10646-013-1074-9"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400046957"},{"key":"e_1_2_7_23_1","first-page":"389","volume-title":"Current Advances in Vitamin K Research","author":"Greaves JH","year":"1988"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2338.1988.tb00403.x"},{"key":"e_1_2_7_25_1","first-page":"89","article-title":"Resistance to the second\u2010 generation anticoagulant rodenticides resistance to the second\u2010generation anticoagulant rodenticides","volume":"11","author":"Lund M","year":"1984","journal-title":"Proc Vertebrate Pest Conf"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400025316"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.2307\/3809478"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.116.071688"},{"key":"e_1_2_7_29_1","volume-title":"Anticoagulant resistance management strategy for pest management professionals, central and local government and other competent users of rodenticides","author":"RRAC","year":"2003"},{"key":"e_1_2_7_30_1","unstructured":"European Medicines Agency Guideline on bioanalytical method validation EMEA\/CHMP\/EWP\/192217\/2009 Rev.1 Corr.* Committee for Medicinal Products for Human Use (CHMP) (2011)."},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2042"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4020"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401645200"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH05-06-0446"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.109.054833"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-122010"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000433"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(99)00038-7"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2004.00301.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.106.011775"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498250110060932"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00266a045"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1506"},{"key":"e_1_2_7_45_1","first-page":"27","article-title":"Efficacy of anticoagulant drugs as rodenticides and genetic variation on Vkorc1 of Mus musculus from Buenos Aires province (Argentina)","volume":"24","author":"Espinosa MB","year":"2013","journal-title":"J Basic Appl Genet"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fps.4797","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.4797","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:47:08Z","timestamp":1761065228000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.4797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,21]]},"references-count":44,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/ps.4797"],"URL":"https:\/\/doi.org\/10.1002\/ps.4797","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,21]]},"assertion":[{"value":"2017-07-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}