{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T07:35:13Z","timestamp":1768030513274,"version":"3.49.0"},"reference-count":69,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T00:00:00Z","timestamp":1518048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Arboviroses such as dengue, Zika and chikungunya represent a serious public health issue as a consequence of the absence of approved vaccines or specific antiviral drugs against the arboviruses that cause them. One way to prevent these diseases is by combating the vector mosquito,\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      (Diptera), which has serine proteases in the midgut. Protease inhibitors are molecules that can block enzyme activity, impairing digestion and nutrition, which can lead to death. Thus, we purified and characterized a novel chymotrypsin\u2010trypsin inhibitor (LsCTI) from\n                      <jats:italic>Lonchocarpus sericeus<\/jats:italic>\n                      seeds and investigated its effect upon\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      egg hatching, larval development and digestive proteases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      LsCTI showed a single protein band in sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis (SDS\u2010PAGE), and the molecular mass determined by matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF\u2010MS) was 8870.45 Da. Kinetics analyses revealed a noncompetitive type of inhibition and low inhibition constant (\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>i<\/jats:sub>\n                      ) for chymotrypsin (8.24 x 10\n                      <jats:sup>\u20108<\/jats:sup>\n                      <jats:sc>m<\/jats:sc>\n                      ). The thermal resistance was remarkable, even at 100 \u00b0C for 180 min. The inhibitor concentration required for 50\u2010percent enzyme inhibition (IC\n                      <jats:sub>50<\/jats:sub>\n                      ) of LsCTI was 4.7 x 10\n                      <jats:sup>\u20107<\/jats:sup>\n                      <jats:sc>m<\/jats:sc>\n                      for\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      midgut larval enzymes. LsCTI did not affect egg hatchability at 0.3 mg mL\n                      <jats:sup>\u20101<\/jats:sup>\n                      , but caused a high larval mortality rate (77%) and delayed development (37%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      LsCTI is a novel protease inhibitor with remarkable biochemical characteristics and is a potential tool to control\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      development. \u00a9 2017 Society of Chemical Industry\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.4812","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T12:24:20Z","timestamp":1511958260000},"page":"1362-1373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["First insights into insecticidal activity against\n                    <i>Aedes aegypti<\/i>\n                    and partial biochemical characterization of a novel low molecular mass chymotrypsin\u2010trypsin inhibitor purified from\n                    <i>Lonchocarpus sericeus<\/i>\n                    seeds"],"prefix":"10.1002","volume":"74","author":[{"given":"Luiz CP","family":"Almeida Filho","sequence":"first","affiliation":[{"name":"Federal University of Cear\u00e1 Biochemistry and Molecular Biology Department  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro MS","family":"Tabosa","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1 Biochemistry and Molecular Biology Department  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise C","family":"Hissa","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1 Biology Department  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilka M","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1 Biochemistry and Molecular Biology Department  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5293-9317","authenticated-orcid":false,"given":"Ana FU","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1 Biology Department  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra064142"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2013.02.021"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1194694"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1603450"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2102.141363"},{"key":"e_1_2_5_7_1","first-page":"311","article-title":"Insect borne diseases transmitted by some important vectors of class insecta hurtling public health. 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