{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:11:19Z","timestamp":1790446279669,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T00:00:00Z","timestamp":1552521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011229","name":"State Key Laboratory of Integrated Pest Management","doi-asserted-by":"publisher","award":["Y852981303"],"award-info":[{"award-number":["Y852981303"]}],"id":[{"id":"10.13039\/501100011229","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Neonicotinoid insecticides are widely used in the prevention and control of pests in agriculture and forestry, but they can also affect the development of nontarget economic insects. In order to determine the effects of trace acetamiprid on the development of reproductive system of silkworm, we studied the gonad development and endogenous hormone metabolism in silkworms exposed to trace acetamiprid.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULT<\/jats:title><jats:p>The silkworm showed mild poisoning symptoms after being exposed to trace acetamiprid (0.01\u2009mg L<jats:sup>\u22121<\/jats:sup>). After 96\u2009h of acetamiprid exposure, the body weight of silkworm was decreased by 7.67% and the ovary and fallopian tube were abnormally developed. The egg production in adults was decreased by 197\u2009\u00b1\u200929, and the egg weight was decreased by 0.52\u2009\u00b1\u20090.01\u2009mg. Quantitative RT\u2010PCR analysis showed that the relative expression levels of ovarian development\u2010related genes <jats:italic>Vg<\/jats:italic>, <jats:italic>Ovo<\/jats:italic>, <jats:italic>Otu<\/jats:italic>, <jats:italic>Sxl\u2010S<\/jats:italic> and <jats:italic>Sxl\u2010L<\/jats:italic> were downregulated by 0.71, 0.77, 0.47, 0.67 and 0.88\u2010fold, respectively. The transcriptional expression of ecdysone metabolism\u2010related gene (<jats:italic>EcR<\/jats:italic>) in the ovary was downregulated by 0.46\u2010fold while the expression of juvenile hormone\u2010related gene (<jats:italic>JHBP2<\/jats:italic>) was upregulated by 1.36\u2010fold. In hemolymph of acetamiprid exposed larvae, the content of ecdysone was reduced whereas the content of juvenile hormone was increased.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Trace acetamiprid may cause reproductive dysfunction in silkworms through regulating the metabolism of endogenous hormones. Our study provides a reference for elucidating the mechanism of acetamiprid\u2010induced reproductive disorders in insects. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5373","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T00:34:35Z","timestamp":1549845275000},"page":"2672-2681","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Mechanism of trace acetamiprid\u2010caused reproductive disorders in silkworm, Bombyx mori"],"prefix":"10.1002","volume":"75","author":[{"given":"Xiaoyu","family":"Cheng","sequence":"first","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanchi","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Mao","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahuan","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6504-2728","authenticated-orcid":false,"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Biological Sciences Soochow University  Suzhou China"},{"name":"School of Basic Medicine and Biological Sciences, Soochow University, Sericulture Institute of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.07.096"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/01480545.2014.966382"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00244-007-9071-8"},{"key":"e_1_2_6_5_1","first-page":"34","article-title":"The toxicity of acetamiprid on silkworm","volume":"44","author":"Shen ZM","year":"2013","journal-title":"Bull Sericul"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2011.12.016"},{"key":"e_1_2_6_7_1","first-page":"644","article-title":"The effect of sublethal doses of insecticides on the functional response of Asecodes hispinarum","volume":"43","author":"Tang C","year":"2006","journal-title":"Chinese Bull Entomol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/03235408.2013.809898"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10646-013-1094-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168042"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-014-0195-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(66)90084-X"},{"key":"e_1_2_6_13_1","first-page":"0094","article-title":"Effects of Acetamiprid on Growth and Development of \n                     Bombyx mori\n                   and Antioxidant Enzyme Activity of Midgut","volume":"44","author":"Zhang MM","year":"2018","journal-title":"Science of Sericulture"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(98)00020-1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2004.03.002"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.09.044"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1.9.913"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.1.123"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.3.897"},{"key":"e_1_2_6_20_1","article-title":"The effect of silencing 20E biosynthesis relative genes by feeding bacterially expressed dsRNA on the larval development of Chilo suppressalis","volume":"6","author":"Jian Z","year":"2016","journal-title":"Sci Rep"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(94)00082-A"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1242\/dev.101.4.931","article-title":"The use of an inhibitor of protein synthesis to investigate the roles of ecdysteroids and sexdetermination genes on the expression of the genes encoding the Drosophila yolk proteins","volume":"101","author":"Bownes M","year":"1987","journal-title":"Development"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00335-5"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/B0-44-451924-6\/00040-5"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-9309(06)80033-5"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2012.08.049"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2018.06.022"},{"key":"e_1_2_6_28_1","first-page":"433","volume-title":"Comprehensive Molecular Insect Science","author":"Raikhel AS","year":"2018"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/154.3.1203"},{"key":"e_1_2_6_30_1","first-page":"133","article-title":"Structural mechanism of JH delivery in hemolymph by JHBP of silkworm","volume":"1","author":"Suzuki R","year":"2011","journal-title":"Bombyx mori Sci Rep"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2008.04.004"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9210"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.14737\/journal.aavs\/2014\/2.10.543.548"},{"key":"e_1_2_6_34_1","first-page":"1","article-title":"Toxic effect of acetamiprid on \n                     Rana ridibunda\n                   sciatic nerve (electrophysiological and histopathological potential)","volume":"14","author":"\u00c7aml\u0131ca Y","year":"2018","journal-title":"Drug Chem Toxicol"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2015.08.009"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.2227"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-014-3332-7"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3783"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fps.5373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.5373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.5373","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.5373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:34:24Z","timestamp":1761064464000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.5373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,14]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/ps.5373"],"URL":"https:\/\/doi.org\/10.1002\/ps.5373","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,14]]},"assertion":[{"value":"2018-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}