{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:49:05Z","timestamp":1790862545570,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008123","name":"Agriculture and Horticulture Development Board","doi-asserted-by":"publisher","award":["CP 162"],"award-info":[{"award-number":["CP 162"]}],"id":[{"id":"10.13039\/100008123","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["1096240"],"award-info":[{"award-number":["1096240"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011102","name":"European Union Seventh Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011262","name":"FP7 Food, Agriculture and Fisheries, Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011262","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The tomato leafminer, <jats:italic>Tuta absoluta<\/jats:italic>, is an economically important pest of tomatoes in Europe, Africa, Asia and South America. In the UK this species is controlled using an integrated pest management (IPM) programme which incorporates the insecticides spinosad and chlorantraniliprole. In response to UK grower concerns of loss of efficacy of these compounds at certain sites, insecticide bioassays were performed on five populations collected from four commercial glasshouses and potential mechanisms of resistance investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>We observed high levels of resistance to spinosad in four of the strains, and in two of these tolerance to chlorantraniliprole. Selection of one of these strains with chlorantraniliprole rapidly resulted in a line exhibiting potent resistance to this compound. Sequencing of messenger RNA encoding the nicotinic acetylcholine receptor (nAChR) \u03b16 subunit, target of spinosad, revealed <jats:italic>Ta\u03b16<\/jats:italic> transcripts in the spinosad\u2010resistant strains that lack exon 4 and encode a highly truncated protein, or contain a triplet deletion in the predicted first transmembrane domain resulting in the loss of a highly conserved amino acid. Sequencing of the ryanodine receptor gene, encoding the target of diamide insecticides, of the chlorantraniliprole\u2010selected line revealed an amino acid substitution (G4903V) that has been previously linked to diamide resistance in populations of <jats:italic>T. absoluta<\/jats:italic> in the Mediterranean and South America.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Taken together our results reveal emerging resistance in UK populations of <jats:italic>T. absoluta<\/jats:italic> to two of the most important insecticides used as part of IPM, with significant implications for the control of this species in the UK. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5381","type":"journal-article","created":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T05:44:52Z","timestamp":1550641492000},"page":"2079-2085","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["The evolution of multiple\u2010insecticide resistance in UK populations of tomato leafminer, <i>Tuta absoluta<\/i>"],"prefix":"10.1002","volume":"75","author":[{"given":"Charles","family":"Grant","sequence":"first","affiliation":[{"name":"College of Life and Environmental Sciences, Biosciences University of Exeter, Penryn Campus  Penryn UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"Jacobson","sequence":"additional","affiliation":[{"name":"RJC Ltd  Bramham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aris","family":"Ilias","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology &amp; Biotechnology Foundation for Research &amp; Technology Hellas  Crete Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Biointeractions and Crop Protection Rothamsted Research  Harpenden UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanouil","family":"Vasakis","sequence":"additional","affiliation":[{"name":"Hellenic Agricultural Organisation\u00a0\u2013\u00a0'Demeter', Institute of Olive Tree Subtropical Crops and Viticulture  Heraklion Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6428-6683","authenticated-orcid":false,"given":"Pablo","family":"Bielza","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Vegetal Universidad Polit\u00e9cnica de Cartagena  Cartagena Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph T","family":"Zimmer","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Biosciences University of Exeter, Penryn Campus  Penryn UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin S","family":"Williamson","sequence":"additional","affiliation":[{"name":"Department of Biointeractions and Crop Protection Rothamsted Research  Harpenden UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard H","family":"ffrench\u2010Constant","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Biosciences University of Exeter, Penryn Campus  Penryn UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Vontas","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology &amp; Biotechnology Foundation for Research &amp; Technology Hellas  Crete Greece"},{"name":"Department of Crop Science Agricultural University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5938-2977","authenticated-orcid":false,"given":"Emmanouil","family":"Roditakis","sequence":"additional","affiliation":[{"name":"Hellenic Agricultural Organisation\u00a0\u2013\u00a0'Demeter', Institute of Olive Tree Subtropical Crops and Viticulture  Heraklion Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2590-1492","authenticated-orcid":false,"given":"Chris","family":"Bass","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Biosciences University of Exeter, Penryn Campus  Penryn UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-011-0398-6"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects4020185"},{"key":"e_1_2_6_4_1","unstructured":"JacobsonRJandHowlettP A robust IPM programme forTuta absoluta. Horticultural Development Company; Factsheet 02\/14. [Online]. Available:https:\/\/horticulture.ahdb.org.uk\/publication\/0214\u2010robust\u2010ipm\u2010programme\u2010tuta\u2010absoluta(2014)."},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-014-0618-y"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2016.02.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2016.04.007"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13882"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2016.11.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-015-0643-5"},{"key":"e_1_2_6_11_1","first-page":"1","article-title":"Field\u2010evolved resistance to chlorantraniliprole in the tomato pinworm Tuta absoluta: inheritance, cross\u2010resistance profile, and metabolism","author":"Silva JE","year":"2019","journal-title":"J Pest Sci"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06924"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.09.001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.06.013"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.09.005"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3404"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.11.009"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000802"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10158-010-0102-1"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.07.010"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2018.05.002"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2018.03.016"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2016.02.001"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2014.04.013"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12029"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1584\/jpestics.D18-007"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10139"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103235"},{"key":"e_1_2_6_29_1","unstructured":"AHDB New Mating Disruption Technique Offers Hope for Tuta Absoluta Control. 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