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In this study, we investigated residues in nine colonies at a model agricultural research site during the period before wintering. Moreover, we applied the acaricide <jats:italic>tau<\/jats:italic>\u2010fluvalinate to the colonies via a strip formulation. The pesticide content was determined by UHPLC\u2010QqQ\u2010MS\/MS in bees from brood comb initially collected in mid\u2010September immediately prior to the start of <jats:italic>tau<\/jats:italic>\u2010fluvalinate treatment and 30 later at the time of <jats:italic>tau<\/jats:italic>\u2010fluvalinate strip removal.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In addition to commonly analyzed pesticides, we detected two plant growth regulators, chlormequat and metazachlor, in the bee colonies. Whereas thiacloprid, chlormequat and acetamiprid decreased after 30\u2009days and contributed considerably to differences between sample time points, other pesticides appeared to be rather stable. Interestingly, we identified diazinon, which has been banned in the European Union since 2007. The residues of methiocarb sulfoxide and imidacloprid\u2010urea in the absence of their parent compounds indicate historical environmental contamination that can be identified by the detection of residues in a bee colony. <jats:italic>tau<\/jats:italic>\u2010Fluvalinate was detected only after the 30\u2010day treatment at an average (\u00b1\u2009SD) concentration of 1.29\u2009\u00b1\u20091.93\u2009ng\/bee, ranging from 0.06 to 7.13\u2009ng\/bee.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The multidimensional behavior of pesticides in a bee colony was indicated. Although the research area is used for agriculture, the measured pesticide level was relatively low. The recorded concentrations of <jats:italic>tau<\/jats:italic>\u2010fluvalinate should not be dangerous to bees, as the values were \u223c\u2009200\u20135000\u2010fold lower than the reported median lethal dose (LD<jats:sub>50<\/jats:sub>) values. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5446","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T01:05:23Z","timestamp":1555290323000},"page":"3245-3251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<i>tau<\/i>\u2010Fluvalinate and other pesticide residues in honey bees before overwintering"],"prefix":"10.1002","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1730-779X","authenticated-orcid":false,"given":"Tomas","family":"Erban","sequence":"first","affiliation":[{"name":"Crop Research Institute  Prague Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Vaclavikova","sequence":"additional","affiliation":[{"name":"ALS Czech Republic ALS Limited  Prague Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Tomesova","sequence":"additional","affiliation":[{"name":"ALS Czech Republic ALS Limited  Prague Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatana","family":"Halesova","sequence":"additional","affiliation":[{"name":"ALS Czech Republic ALS Limited  Prague Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0740-166X","authenticated-orcid":false,"given":"Jan","family":"Hubert","sequence":"additional","affiliation":[{"name":"Crop Research Institute  Prague Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1603\/EC10235"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054092"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido\/2010018"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094482"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3896\/IBRA.1.51.1.12"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009754"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215039"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029268"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13592-011-0011-2"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-0493-93.2.189"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects9020055"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-0493-95.1.28"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.086538"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218839.1998.11100956"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:2005043"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.303"},{"key":"e_1_2_6_18_1","first-page":"50","article-title":"Fluvalinate residues in Greek honey and beeswax","volume":"38","author":"Tsigouri AD","year":"2003","journal-title":"Apiacta"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b02813"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218839.2001.11101054"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006379114942"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-016-0023-8"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:19990211"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:19950507"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:19970605"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218839.2006.11101326"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218839.2002.11101064"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.08.006"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3679"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-018-0968-y"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155332"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082941"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:19970206"},{"key":"e_1_2_6_34_1","unstructured":"BRI.GABON PF 90 mg prouzky do ulu: pribalova informace [GABON PF 90 mg hive strips: package leaflet]. 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