{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:31:10Z","timestamp":1762299070671,"version":"build-2065373602"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Aspergillus fumigatus<\/jats:italic>, the causal agent of aspergillosis in humans, is commonly present as a saprophyte in various organic substrates, such as spoiled silages. Aspergillosis is generally combated with demethylation inhibitor (DMI) fungicides, but the recent appearance of resistant medical and environmental strains made current treatment strategies less reliable. The goal of this study was to determine the evolution of <jats:italic>A. fumigatus<\/jats:italic> populations during the ensiling process of whole\u2010crop corn, high moisture corn and wet grain corn, and to monitor the sensitivity of isolates from treated and untreated fields to one medical and one agricultural DMI fungicide.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:italic>A. fumigatus<\/jats:italic> was isolated from fresh forage at harvest at rather low concentrations (10<jats:sup>2<\/jats:sup>\u2009cfu\u2009g<jats:sup>\u22121<\/jats:sup>). The low frequency lingered during the silage process (at 60 and 160\u2009days), whereas it significantly increased during air exposure (at 7 and 14\u2009days of air exposure). Field treatment of corn with a mixture of prothioconazole and tebuconazole did not affect the sensitivity of <jats:italic>A. fumigatus<\/jats:italic> isolates. One of 29 isolates from the untreated plot was resistant to voriconazole. A unique amino acid substitution (E427K) was detected in the <jats:italic>cyp51A<\/jats:italic> gene of 10 of 12 sequenced isolates, but was not associated with DMI resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:italic>A. fumigatus<\/jats:italic> significantly increased during aerobic deterioration of ensilaged corn after silo opening, compared with the low presence in fresh corn and during ensiling. Field treatment of corn with DMI fungicides did not affect the sensitivity of <jats:italic>A. fumigatus<\/jats:italic> isolates collected from fresh and ensiled corn. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5566","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T06:53:31Z","timestamp":1564124011000},"page":"685-694","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Aspergillus fumigatus<\/i> population dynamics and sensitivity to demethylation inhibitor fungicides in whole\u2010crop corn, high moisture corn and wet grain corn silages"],"prefix":"10.1002","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5207-9345","authenticated-orcid":false,"given":"Davide","family":"Spadaro","sequence":"first","affiliation":[{"name":"AGROINNOVA \u2013 Centre of Competence for the Innovation in the Agro\u2010environmental Sector Universit\u00e0 di Torino  Grugliasco Italy"},{"name":"Dept. 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