{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T02:49:58Z","timestamp":1788058198735,"version":"build-2784847793"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T00:00:00Z","timestamp":1571961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ01182003"],"award-info":[{"award-number":["PJ01182003"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Insulin\/insulin\u2010like growth factor signaling (IIS) is known to mediate larval growth and adult reproduction in the legume pod borer, <jats:italic>Maruca vitrata<\/jats:italic> (Lepidoptera: Crambidae). Four IIS components (InR, FOXO, Akt, and TOR) play crucial roles in the IIS pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>RNA interference (RNAi) against any of these four IIS component genes was effective in suppressing each target mRNA level by either hemocoelic injection or oral administration using gene\u2010specific double\u2010stranded RNAs (dsRNAs). These RNAi treatments interfered with larval growth, leading to small pupae or significant larval mortality. For massive production of dsRNA, transformed bacteria expressing dsRNAs of these four IIS components were prepared with L4440 expression vector and HT115 strain of <jats:italic>Escherichia coli<\/jats:italic>. The transformed bacteria killed the larvae in a dose\u2010dependent manner by feeding administration. An ultra\u2010sonication pretreatment was performed to impair bacterial membrane and increase dsRNA release from the bacteria in insect intestine. This pretreatment increased the insecticidal activity of these recombinant bacteria. To further increase dsRNA toxicity, its mixture with <jats:italic>Bacillus thuringiensis<\/jats:italic> (Bt) was prepared and showed significant increase of Bt insecticidal activity in the laboratory. The bacterial mixture also showed a high control efficacy (83.3%) in an adzuki bean (<jats:italic>Vigna angularis<\/jats:italic>) field infested by <jats:italic>M. vitrata<\/jats:italic>. Furthermore, such a dsRNA effect was specific for <jats:italic>M. vitrata<\/jats:italic>, but not for non\u2010target insects.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The bacteria expressing dsRNA specific to IIS components can be used to develop dsRNA insecticide. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5612","type":"journal-article","created":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T10:43:15Z","timestamp":1568112195000},"page":"1020-1030","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Alteration of insulin signaling to control insect pest by using transformed bacteria expressing dsRNA"],"prefix":"10.1002","volume":"76","author":[{"given":"Abdullah","family":"Al Baki","sequence":"first","affiliation":[{"name":"Department of Plant Medicals Andong National University  Andong South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Kyo","family":"Jung","sequence":"additional","affiliation":[{"name":"Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science National Institute of Crop Science, Rural Development Administration  Suwon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6840-2167","authenticated-orcid":false,"given":"Yonggyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plant Medicals Andong National University  Andong South 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