{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:52:24Z","timestamp":1786978344283,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The application of RNA interference (RNAi) technique in controlling agricultural insect pests has been receiving much attention since the discovery of RNAi. The brown planthopper (BPH)\n                      <jats:italic>Nilaparvata lugens<\/jats:italic>\n                      , a notorious pest of rice, has evolved a high level of resistance to many kinds of insecticides.\n                      <jats:italic>Tyrosine hydroxylase<\/jats:italic>\n                      (\n                      <jats:italic>Th<\/jats:italic>\n                      ) is an indispensable survival gene in holometabolous insects, playing key roles in cuticle tanning and immunity. In this study, we investigated whether\n                      <jats:italic>Th<\/jats:italic>\n                      could be used as a potential target in controlling\n                      <jats:italic>N. lugens<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Here, we demonstrated that\n                      <jats:italic>NlTh<\/jats:italic>\n                      had a periodical expression pattern during molting with the highest level observed in epidermis. Dysfunction of NlTH by dsNlTh microinjection or 3\u2010IT feeding similarly caused rapid death of\n                      <jats:italic>N. lugens<\/jats:italic>\n                      . Compared with dsGFP control BPHs, dsNlTh injected BPHs (i) had cuticle pigmentation and sclerotizaton defects; (ii) had less endocuticle lamella in tergum integument; (iii) showed higher mortality during the molting process as a result of defective cuticle shedding; (iv) showed feeding disorders indicated by a low number of probe wound dots on rice; (v) had more vulnerable cuticle.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This study demonstrated that TH orthologues play a conservative and crucial role for exocuticle tanning in both holometabolous and hemimetabolous insects, and\n                      <jats:italic>NlTh<\/jats:italic>\n                      could be targeted for RNAi\u2010mediated BPH control. The rapid lethal phenotype of NlTH dysfunction BPHs partly induced by cuticle formation defects. \u00a9 2020 Society of Chemical Industry\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.5760","type":"journal-article","created":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T01:40:00Z","timestamp":1579916400000},"page":"2225-2232","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["RNA interference of tyrosine hydroxylase caused rapid mortality by impairing cuticle formation in\n                    <i>Nilaparvata lugens<\/i>\n                    (Hemiptera: Delphacidae)"],"prefix":"10.1002","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5286-2951","authenticated-orcid":false,"given":"Shu H","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology China National Rice Research Institute  Hangzhou 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