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Odourant\u2010binding proteins (OBPs) play an important role in the olfactory perception of insects. Revealing the function of DcitOBPs is beneficial to the development of new ACP management strategies.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>An analysis of the components of volatiles from the new shoots of six host plant species showed that \u03b2\u2010caryophyllene was the characteristic volatile compound in flushing shoots and the most abundant volatile compound in three of the six tested ACP host plant species. The tissue expression profiles of nine known DcitOBPs were analyzed based on a transcriptome database, and <jats:italic>DcitOBP3<\/jats:italic> and <jats:italic>DcitOBP6<\/jats:italic> exhibited high expression in the antennae of both sexes and the ovipositor of females. The binding ability of two recombinant proteins with eight ligands was studied through competitive binding analysis; the results showed that <jats:italic>DcitOBP6<\/jats:italic> exhibited stronger binding to \u03b2\u2010caryophyllene. Behavioural trials indicated that sexually mature female adults of <jats:styled-content style=\"fixed-case\"><jats:italic>D. citri<\/jats:italic><\/jats:styled-content> were significantly attracted to \u03b2\u2010caryophyllene at concentrations of 0.1 \u03bcL\u2009mL<jats:sup>\u22121<\/jats:sup> and 10 \u03bcL\u2009mL<jats:sup>\u22121<\/jats:sup>. RNAi analysis in female <jats:styled-content style=\"fixed-case\"><jats:italic>D. citri<\/jats:italic><\/jats:styled-content> showed that the reduction of <jats:italic>DcitOBP6<\/jats:italic> transcript abundance led to a decrease in antennae EAG activity and behavioural responses to \u03b2\u2010caryophyllene.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The results demonstrate that <jats:italic>DcitOBP6<\/jats:italic> is involved in the perception of an important host plant volatile, \u03b2\u2010caryophyllene, in the ACP, and provide a theoretical foundation for behavioural interference in ACP management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5786","type":"journal-article","created":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T08:35:33Z","timestamp":1581669333000},"page":"2453-2464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Host plant odours and their recognition by the odourant\u2010binding proteins of <scp><i>Diaphorina citri<\/i><\/scp> Kuwayama (Hemiptera: Psyllidae)"],"prefix":"10.1002","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4460-0369","authenticated-orcid":false,"given":"Zhengbing","family":"Wang","sequence":"first","affiliation":[{"name":"Guangdong Engineering Research Center for Insect Behavior Regulation; Key Laboratory of Bio\u2010Pesticide Innovation and Application College of Agriculture, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanhe","family":"Gao","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Research Center for Insect Behavior Regulation; Key Laboratory of Bio\u2010Pesticide Innovation and Application College of Agriculture, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Research Center for Insect Behavior Regulation; Key Laboratory of Bio\u2010Pesticide Innovation and Application College of Agriculture, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwu","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology; Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Insect Sciences, Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2979-4305","authenticated-orcid":false,"given":"Xinnian","family":"Zeng","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Research Center for Insect Behavior Regulation; Key Laboratory of 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