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Our group has previously synthesized and screened the antimicrobial activity of diverse 1,3,4\u2010oxadiazole thioether\/sulfone compounds bridged by a sulfur atom at the 2\u2010position of 1,3,4\u2010oxadiazole. However, few studies have evaluated the effect of eliminating the sulfur atom on bioactivity. Herein, a novel type of <jats:italic>N<\/jats:italic>\u2010containing heterocyclic pendants\u2010tagged 1,3,4\u2010oxadiazoles bridged by alkyl chains only was systematically synthesized and evaluated for their antimicrobial activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Bioassay results revealed that antibacterial efficacy increased by 551\u2010 and 314\u2010fold against the corresponding phytopathogens <jats:italic>Xanthomonas oryzae<\/jats:italic> pv. <jats:italic>oryzae<\/jats:italic> and <jats:italic>X. axonopodis<\/jats:italic> pv. <jats:italic>citri<\/jats:italic> compared to commercial agents bismerthiazol and thiodiazole copper. <jats:italic>In vivo<\/jats:italic> trials showed that <jats:bold>C<\/jats:bold><jats:sub>\n<jats:bold>1<\/jats:bold><\/jats:sub> exerted remarkable curative activity against rice bacterial blight with a control effectiveness of 52.9% at 200\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>. Antibacterial mechanism research found that <jats:bold>C<\/jats:bold><jats:sub>\n<jats:bold>1<\/jats:bold><\/jats:sub> could reduce the hypersensitive response behavior and pathogenicity of <jats:italic>Xoo<\/jats:italic> through targeting the type III secretion system (T3SS) at a lower drug dose. This outcome was verified by observing the significantly down\u2010regulated proteins and representative genes from the related quantitative proteomics and qRT\u2010PCR assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study can inspire the design of innovative molecular frameworks targeting the T3SS of phytopathogens for controlling bacterial infections. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5814","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T06:41:05Z","timestamp":1583736065000},"page":"2681-2692","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Design, synthesis, and antimicrobial behavior of novel oxadiazoles containing various <i>N<\/i>\u2010containing heterocyclic pendants"],"prefix":"10.1002","volume":"76","author":[{"given":"Dan","family":"Zeng","sequence":"first","affiliation":[{"name":"State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Xiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key 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