{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:39:43Z","timestamp":1788611983085,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Project of Shanghai Science and Technology Innovation Action Plan","award":["15\u2009431\u2009906\u2009800"],"award-info":[{"award-number":["15\u2009431\u2009906\u2009800"]}]},{"name":"Project of Shanghai Science and Technology Innovation Action Plan","award":["19391902400"],"award-info":[{"award-number":["19391902400"]}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Sclerotinia stem rot (SSR) caused by <jats:italic>Sclerotinia sclerotiorum<\/jats:italic> threatens oilseed rape cultivation, and the emergence of fungicide\u2010resistant strains has led to control failures worldwide. Identifying novel chemical alternatives with different modes of action and high antifungal activities is thus crucial. Herein we evaluated the antifungal effects of 3\u2010(2\u2010pyridyl)methyl\u20102\u2010(4\u2010chlorphenyl)imino\u2010 thiazolidine (PMAS) on <jats:italic>S. sclerotiorum<\/jats:italic> to determine its efficacy for SSR management.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>PMAS had an inhibitory effect on mycelial growth; the EC<jats:sub>50<\/jats:sub> values were 17.83 and 21.15\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup> for the carbendazim\u2010susceptible strain Ss01 and carbendazim\u2010resistant strain Hm25, respectively. PMAS treatment changed the color of inhibited mycelia to green, and the hyphae were sustained in the undifferentiated stage. Cysteine supplementation made this green color disappear, whereas methionine enhanced the color. Moreover, PMAS treatment markedly inhibited oxalic acid biogenesis, increased free thiol content in mycelia, and weakened the activities of oxaloacetase and malate dehydrogenase, but had little effect on the activity of glyoxylate dehydrogenase. Cysteine could reverse the inhibitory effects of PMAS on mycelial morphogenesis and biochemical constituents, except thiol production. In the pot\u2010culture experiment, PMAS showed a good protective effect, with the control efficacy being &gt;91% on SSR.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>PMAS appears to be an effective fungicide for SSR management. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.5843","type":"journal-article","created":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T04:25:19Z","timestamp":1585974319000},"page":"2978-2985","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Antifungal effects of 3\u2010(2\u2010pyridyl)methyl\u20102\u2010(4\u2010chlorphenyl) iminothiazolidine against <i>Sclerotinia sclerotiorum<\/i>"],"prefix":"10.1002","volume":"76","author":[{"given":"Xianfei","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Chemical Biology, School of Pharmacy East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuyong","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Chemical Biology, School of Pharmacy East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solange","family":"Muhayimana","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Chemical Biology, School of Pharmacy East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xiong","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Chemical Biology, School of Pharmacy East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Chemical Biology, School of Pharmacy East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1731-8384","authenticated-orcid":false,"given":"Qingchun","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Chemical Biology, School of Pharmacy East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-69-875"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/07060669409500766"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2005.00316.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-204"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-06-18-0197-RVW"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2171"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2007.01.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4051"},{"key":"e_1_2_6_10_1","first-page":"493","volume-title":"Molecular Methods in Plant Pathology. 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