{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:31:56Z","timestamp":1761093116733,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2002,10,11]],"date-time":"2002-10-11T00:00:00Z","timestamp":1034294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Conseil Regional de Bourgogne"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nutrient\u2010agar plates containing isoxaben (500\u2009mg\u2009litre<jats:sup>\u22121<\/jats:sup>) were used to isolate isoxaben\u2010metabolising bacteria from four European soils incubated with the herbicide under laboratory conditions. In flask experiments, inoculation of a basal salts medium containing nitrogen and [<jats:italic>phenyl<\/jats:italic>\u2010U\u2010<jats:sup>14<\/jats:sup>C]isoxaben with an isolate (B2b) resulted in 33% recovery of the initial radioactivity as [<jats:sup>14<\/jats:sup>C]carbon dioxide after 2 weeks. A major metabolite identified by GC\u2013MS and NMR analysis as 3\u2010(1\u2010ethyl\u20101\u2010methylpropyl)isoxazol\u20105\u2010ylamine accumulated both in basal salts and nutrient broth media. 2,6\u2010Dimethoxybenzoic acid, a suspected metabolite of isoxaben, was not detected in either liquid media. However, the capability of the B2b isolate to use 2,6\u2010dimethoxybenzoic acid as a source of carbon was demonstrated. Soil inoculation with the B2b strain resulted in an increase in the recovery of [<jats:sup>14<\/jats:sup>C]carbon dioxide from both [<jats:italic>phenyl<\/jats:italic>\u2010U\u2010<jats:sup>14<\/jats:sup>C] and [<jats:italic>isoxazole<\/jats:italic>\u20105\u2010<jats:sup>14<\/jats:sup>C]isoxaben. The metabolite identified as 3\u2010(1\u2010ethyl\u20101\u2010methylpropyl)isoxazole\u20105\u2010ylamine only accumulated if the soil was autoclaved before inoculation. This metabolite was rapidly mineralized by the microflora of a natural soil without history of isoxaben treatment. Homology patterns of sequenced 16S rDNA between isoxaben\u2010transforming isolates and reference strains showed that the four isolates identified belonged to the genus <jats:italic>Microbacterium<\/jats:italic>.<\/jats:p><jats:p>\u00a9 2002 Society of Chemical Industry<\/jats:p>","DOI":"10.1002\/ps.599","type":"journal-article","created":{"date-parts":[[2002,11,15]],"date-time":"2002-11-15T10:54:30Z","timestamp":1037357670000},"page":"1229-1235","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Isolation and characterization of efficient isoxaben\u2010transforming <i>Microbacterium<\/i> sp strains from four European 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