{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T16:35:57Z","timestamp":1770741357969,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T00:00:00Z","timestamp":1600992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Bacillus velezensis<\/jats:italic> and other <jats:italic>Bacillus<\/jats:italic> species are known for the production of bioactive secondary metabolites that are active against plant pathogenic fungi. In view of this, a study was undertaken to investigate the antifungal potential of <jats:italic>B. velezensis<\/jats:italic> AR1 culture supernatant.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULT<\/jats:title><jats:p>The butanol extract concentrate of <jats:italic>B. velezensis<\/jats:italic> AR1 was separated into different fractions by column chromatography. A fraction eluted by 9:1 chloroform: methanol caused 25.8\u201370.2% and 25.2\u201356.3% growth inhibition of <jats:italic>Monilinia fructicola<\/jats:italic> and <jats:italic>Colletotricum goeosporioides<\/jats:italic>, respectively. This fraction was subjected to solid\u2010phase extraction using a Strata SI\u20101 column and further purified by prep\u2010TLC to obtain a pure metabolite showing a single peak on high performance liquid chromatography. On the basis of the nuclear magnetic resonance (NMR <jats:sup>1<\/jats:sup>H, <jats:sup>13<\/jats:sup>C, H\uf8ffH COSY, HSQC and HMBC) analysis, the metabolite was identified as 5\u2010<jats:italic>N<\/jats:italic>\u2010tyrosinylornithine. The compound exhibited antifungal activity against two plant pathogenic fungi.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>5\u2010<jats:italic>N<\/jats:italic>\u2010tyrosinylornithine, the secondary metabolite isolated from the culture supernatant of <jats:italic>B. velezensis<\/jats:italic> AR1 exhibited significant antifungal activity against two plant pathogenic fungi.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6078","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T04:04:29Z","timestamp":1599797069000},"page":"775-779","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Purification and identification of a new antifungal dipeptide from <scp><i>Bacillus velezensis<\/i> AR1<\/scp> culture supernatant"],"prefix":"10.1002","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7124-385X","authenticated-orcid":false,"given":"Regassa A","family":"Bayisa","sequence":"first","affiliation":[{"name":"Institute of Applied Research, College of Agriculture and Environmental Sciences Arsi University  Asella Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong Yong","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Food Engineering Chonnam National University  Gwangju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kil Yong","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences Chonnam National University  Gwangju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9GC00457B"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35012241"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01286"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/np300024u"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-016-2176-5"},{"key":"e_1_2_7_7_1","first-page":"507","volume-title":"Probiotics and Plant Health","author":"Khasa YP","year":"2017"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2016.01.013"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2018.03.002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25346-3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2013.765414"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-015-1708-x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1804.04025"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13313-020-00701-z"},{"key":"e_1_2_7_15_1","article-title":"Enhancing resistance of Sesamum indicum against Alternaria sesami through Bacillus velezensis AR1","author":"Regassa AB","year":"2020","journal-title":"Pest Manag Sci"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2016.06.002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.5423\/PPJ.OA.04.2017.0073"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22782-z"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2018.11.020"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/biol-2019-0046"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B1600117"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213-013-0632-y"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-76-136"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-014-3520-7"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fps.6078","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.6078","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.6078","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.6078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T11:37:22Z","timestamp":1761046642000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.6078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,25]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/ps.6078"],"URL":"https:\/\/doi.org\/10.1002\/ps.6078","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,25]]},"assertion":[{"value":"2020-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}