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Here, we investigated the resistance levels of a T. urticae<\/jats:italic> population collected from an ornamental greenhouse in Peloponnese, Greece, and analyzed its resistance mechanisms at the molecular level.<\/jats:p><\/jats:sec>RESULTS<\/jats:title>Toxicological assays showed resistance against compounds with different modes of action, with resistance ratios of: 89\u2010fold for abamectin; >\u20091000\u2010fold for clofentezine; >\u20095000\u2010fold for etoxazole; 27\u2010fold for fenpyroximate and pyridaben; 20\u2010 and 36\u2010fold for spirodiclofen and spirotetramat, respectively; and 116\u2010 and >\u2009500\u2010fold for cyenopyrafen and cyflumetofen, respectively.<\/jats:p>Bioassays with synergists indicated the involvement of detoxification enzymes in resistance to abamectin, but not to cyflumetofen and spirodiclofen. RNA sequencing (RNA\u2010seq) analysis showed significant over\u2010expression of several genes encoding detoxification enzymes such as cytochrome P450 monooxygenases and UDP\u2010glycosyltransferases, which have been previously associated with acaricide resistance. Known target\u2010site resistance mutations were identified in acetyl\u2010choline esterase<\/jats:italic>, chitin synthase 1<\/jats:italic> and NDUFS7\/psst<\/jats:italic>, but putative novel resistance mutations were also discovered in targets such as glutamate\u2010gated chloride channel subunit 3<\/jats:italic>. Interestingly, target\u2010site resistance mutations against pyrethroids or bifenazate were not identified, possibly indicating a recent reduced selection pressure in Greece, as well as a possible opportunity to rotate these chemistries.<\/jats:p><\/jats:sec>CONCLUSION<\/jats:title>We identified and characterized a striking case of multiple acaricide resistance in a field population of T. urticae<\/jats:italic>. Exceptionally strong resistance phenotypes, with accumulation of multiple resistance mutations and over\u2010expression of P450s and other detoxification genes in the same field population are reported.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6136","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T22:06:12Z","timestamp":1602626772000},"page":"666-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Identification and characterization of striking multiple\u2010insecticide resistance in a Tetranychus urticae<\/i> field population from Greece"],"prefix":"10.1002","volume":"77","author":[{"given":"Kyriaki Maria","family":"Papapostolou","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology & Biotechnology, Foundation of Research & Technology Hellas Heraklion Greece"},{"name":"Department of Biology University of Crete Heraklion 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