{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:39:21Z","timestamp":1788320361489,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T00:00:00Z","timestamp":1605744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M663195"],"award-info":[{"award-number":["2019M663195"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The fall armyworm <jats:italic>Spodoptera frugiperda<\/jats:italic> is a major agricultural pest that has invaded the East Hemisphere since 2016, generating a serious threat to food security worldwide including Africa and Asia. The Cry toxins produced by <jats:italic>Bacillus thuringiensis<\/jats:italic> (Bt) have been shown to be effective against this insect pest. In different insect ABC transporters (ABCC2 or ABCC3) have been shown to be involved as receptors of some Cry1 toxins. Here we analyzed the role of SfABCC2 and SfABCC3 in the toxicity of Cry1Fa and Cry1Ab toxins in this insect pest.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Two <jats:italic>S. frugiperda SfABCC2<\/jats:italic> and <jats:italic>SfABCC3<\/jats:italic> knockout strains, coding for potential functional Bt receptors, were created using CRISPR\/Cas9 genome editing system. Both knockout strains showed resistance to both Cry1Fa and Cry1Ab toxins compared with the susceptible strain. SfABCC2 knockout strain showed higher resistance to both Cry toxins than SfABCC3 knockout strain, suggesting a major role of SfABCC2 in the mode of action of these Cry toxins. In addition, expression of <jats:italic>SfABCC2<\/jats:italic> and <jats:italic>SfABCC3<\/jats:italic> genes in <jats:italic>Trichoplusia ni<\/jats:italic> Hi5 cells also increased the susceptibility to Cry1Ab and Cry1Fa toxins, in agreement with the genome editing results. The double knockout of <jats:italic>SfABCC2<\/jats:italic> and <jats:italic>SfABCC3<\/jats:italic> strain was not viable in contrast to other lepidopteran species. Furthermore, we report here that <jats:italic>SfABCC2<\/jats:italic> or <jats:italic>SfABCC3<\/jats:italic> knockout strains increased their susceptibility to abamectin and spinosad insecticides.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>We provide functional evidence that in <jats:italic>S. frugiperda<\/jats:italic> these two ABCC transporters serve as receptors of Bt Cry1Fa and Cry1Ab toxins. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6170","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T08:32:13Z","timestamp":1604478733000},"page":"1492-1501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Two <scp>ABC<\/scp> transporters are differentially involved in the toxicity of two <scp><i>Bacillus thuringiensis<\/i><\/scp> Cry1 toxins to the invasive crop\u2010pest <scp><i>Spodoptera frugiperda<\/i><\/scp> (J. E. Smith)"],"prefix":"10.1002","volume":"77","author":[{"given":"Minghui","family":"Jin","sequence":"first","affiliation":[{"name":"Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences  Shenzhen China"},{"name":"The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanchao","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinxue","family":"Shan","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7096-5690","authenticated-orcid":false,"given":"Swapan","family":"Chakrabarty","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cheng","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Sober\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico  Cuernavaca Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Bravo","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico  Cuernavaca Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyu","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3555-4292","authenticated-orcid":false,"given":"Kongming","family":"Wu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3203-9375","authenticated-orcid":false,"given":"Yutao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"e0175076","DOI":"10.1371\/journal.pone.0175076","article-title":"Fall armyworm migration across the Lesser Antilles and the potential for genetic exchanges between north and south American populations","volume":"12","author":"Nagoshi RN","year":"2017","journal-title":"Plos One"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165632"},{"key":"e_1_2_7_4_1","article-title":"Case study on the first immigration of fall armyworm Spodoptera frugiperda invading into China","author":"Sun XX","year":"2020","journal-title":"J Integr Agr"},{"key":"e_1_2_7_5_1","first-page":"1","article-title":"Management strategies of fall armyworm (Spodoptera frugiperda) in China","volume":"46","author":"Wu KM","year":"2020","journal-title":"Plant Protect"},{"key":"e_1_2_7_6_1","unstructured":"FAO. 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