{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T05:27:26Z","timestamp":1771910846875,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T00:00:00Z","timestamp":1613865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2021,6]]},"abstract":"Abstract<\/jats:title>BACKGROUND<\/jats:title>Tenvermectin A is a new avermectin derivative that has good insecticidal and acaricidal effects. In order to study the resistance of Plutella xylostella<\/jats:italic> to tenvermectin A, a sensitive strain (SS) and a laboratory\u2010selected tenvermectin A\u2010resistant strain (RS, 33.57\u2010fold) were used to evaluate cross\u2010resistance and fitness costs as well as to determine the resistance mechanism.<\/jats:p><\/jats:sec>RESULTS<\/jats:title>There was no cross\u2010resistance with common pesticides except for moderate cross\u2010resistance with cypermethrin (resistance ratio = 10.26\u2010fold) observed in RS. The activities of metabolic enzymes were measured, and the results showed that mixed function oxidase (MFO) and carboxylate esterase (CarE) in RS increased significantly by 2.92\u2010 and 2.86\u2010fold, respectively, compared with SS. In addition, there was no obvious difference in glutathione\u2010S\u2010transferase (GST), which indicated that enhanced MFO and CarE activities may be the main mechanisms of detoxification. In the four typical resistance\u2010related genes, expression of GluCl<\/jats:italic> (4.86\u2010fold), ABCC2<\/jats:italic> (3.85\u2010fold), and CYP6<\/jats:italic> (2.94\u2010fold) in RS were significantly promoted, but expression of GST<\/jats:italic> was not. The clone and sequence of the Px<\/jats:italic>GluCl\u03b1 subunit displayed six mutations that could lead to changes in the amino acid residues.<\/jats:p><\/jats:sec>CONCLUSION<\/jats:title>High suitability related to tenvermectin A resistance was observed in RS, and it was found that the developmental stages of RS were significantly shortened and the survival rate of females was reduced. In addition, the mechanism of resistance to tenvermectin A may be regulated by the glutamate\u2010gated chloride channel, ATP\u2010binding cassette transporter, and MFO. In general, the study of resistance and biochemical mechanisms can provide beneficial and rational information for the management of resistance in P. xylostella<\/jats:italic>.<\/jats:p>\u00a9 2021 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6317","type":"journal-article","created":{"date-parts":[[2021,2,7]],"date-time":"2021-02-07T06:55:24Z","timestamp":1612680924000},"page":"2826-2835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Cross\u2010resistance, fitness costs, and biochemical mechanism of laboratory\u2010selected resistance to tenvermectin A<\/scp> in Plutella xylostella<\/i>"],"prefix":"10.1002","volume":"77","author":[{"given":"Linying","family":"Zhu","sequence":"first","affiliation":[{"name":"Collaborative Innovation Center of Green Pesticide, National Joint Engineering Laboratory of Biopesticide Preparation Zhejiang A&F University State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science, Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province Huzhou University Huzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Lu","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center of Green Pesticide, National Joint Engineering Laboratory of Biopesticide Preparation Zhejiang A&F University State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center of Green Pesticide, National Joint Engineering Laboratory of 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