{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T22:16:01Z","timestamp":1784585761240,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T00:00:00Z","timestamp":1614211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Aedes aegypti<\/jats:italic> is a remarkably effective mosquito vector of epidemiologically important arboviral diseases including dengue fever, yellow fever and Zika. The present spread of resistance against pyrethroids, the primary insecticides used for mosquito control, in global populations of this species is of great concern. The voltage\u2010gated sodium channel (VGSC) in the nervous system is the known target site of pyrethroids in insects. Past studies have revealed several amino\u2010acid substitutions in this channel that confer pyrethroid resistance, which are known as knockdown resistance (<jats:italic>kdr<\/jats:italic>) mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>This study investigated a laboratory colony of <jats:italic>Ae. aegypti<\/jats:italic>, MCNaeg, established from larvae collected in Rio de Janeiro, Brazil in 2016. The MCNaeg colony showed strong resistance against pyrethroids without laboratory selection. Of the two <jats:italic>VGSC<\/jats:italic> gene haplotypes present within this colony, one harbored three known <jats:italic>kdr<\/jats:italic> mutations, V410L, V1016I, and F1534C, and the other harbored only the known F1534C mutation. In latter haplotype, we also found novel amino\u2010acid substations including V253F. Previous molecular modeling and electrophysiological studies suggest that this residue serves a pyrethroid\u2010sensing site in the second receptor, PyR2. Our genetical analysis showed that the haplotype harboring V253F and F1534C is associated with equal or slightly stronger resistance than the other triple <jats:italic>kdr<\/jats:italic> haplotype to both Type I and Type II pyrethroids.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The novel substitution V253F is potentially involved in pyrethroid resistance in <jats:italic>Ae. aegypti<\/jats:italic>. Further studies are needed to elucidate the role of this substitution in the pyrethroid susceptibility of VGSC.<\/jats:p><jats:p>\u00a9 2021 The Authors. <jats:italic>Pest Management Science<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6324","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T01:02:16Z","timestamp":1612918936000},"page":"2887-2893","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["A first, naturally occurring substitution at the second pyrethroid receptor of voltage\u2010gated sodium channel of <scp><i>Aedes aegypti<\/i><\/scp>"],"prefix":"10.1002","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1300-9883","authenticated-orcid":false,"given":"Kentaro","family":"Itokawa","sequence":"first","affiliation":[{"name":"Pathogen Genomics Center National Institute 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