{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T22:45:39Z","timestamp":1776638739827,"version":"3.51.2"},"reference-count":63,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Tomato chlorosis virus (ToCV) is mainly vectored by <jats:italic>Bemisia tabaci<\/jats:italic> in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the <jats:italic>B. tabaci Bta11975<\/jats:italic> gene, an <jats:italic>\u03b1<\/jats:italic>\u2010glucosidase (<jats:italic>AGLU<\/jats:italic>) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of <jats:italic>Bta11975<\/jats:italic> gene in ToCV acquisition and transmission by <jats:italic>B. tabaci<\/jats:italic> MED, we used RNA interference (RNAi) to reduce the expression of the <jats:italic>Bta11975<\/jats:italic> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The relative expression of the <jats:italic>Bta11975<\/jats:italic> gene was correlated with the ToCV content in <jats:italic>B. tabaci<\/jats:italic>. The <jats:italic>AGLU<\/jats:italic> is highly expressed in primary salivary gland and gut. After the <jats:italic>Bta11975<\/jats:italic> gene was silenced, the gene expression of <jats:italic>B. tabaci<\/jats:italic> was reduced and <jats:italic>B. tabaci<\/jats:italic> mortality was increased. Besides, ToCV acquisition by <jats:italic>B. tabaci<\/jats:italic> at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of <jats:italic>B. tabaci<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>These results indicate that suppression of expression of the <jats:italic>Bta11975<\/jats:italic> gene in <jats:italic>B. tabaci<\/jats:italic> MED by RNAi can reduce acquisition and transmission of ToCV by <jats:italic>B. tabaci<\/jats:italic> MED.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6572","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T11:42:56Z","timestamp":1627299776000},"page":"5294-5303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Suppression of <i>Bta11975<\/i>, an <i>\u03b1\u2010<\/i>glucosidase, by <scp>RNA<\/scp> interference reduces transmission of tomato chlorosis virus by <scp><i>Bemisia tabaci<\/i><\/scp>"],"prefix":"10.1002","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5922-0110","authenticated-orcid":false,"given":"DingYiHui","family":"Lu","sequence":"first","affiliation":[{"name":"Subcollege of Longping Graduate School of Hunan University  Changsha China"},{"name":"Institute of Plant Protection Hunan Academy of Agricultural Sciences  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Yue","sequence":"additional","affiliation":[{"name":"Subcollege of Longping Graduate School of Hunan University  Changsha China"},{"name":"Institute of Plant Protection Hunan Academy of Agricultural Sciences  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LiPing","family":"Huang","sequence":"additional","affiliation":[{"name":"Subcollege of Longping Graduate School of Hunan University  Changsha China"},{"name":"Institute of Plant Protection Hunan Academy of Agricultural 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