{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:55:48Z","timestamp":1785894948870,"version":"3.56.0"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Blood\u2010sucking insects incorporate many times their body weight of blood in a single meal. Because proteins are the major component of vertebrate blood, its digestion in the gut generates extremely high concentrations of free amino acids. Previous reports showed that the tyrosine degradation pathway plays an essential role in adapting these animals to blood feeding. Inhibition of 4\u2010hydroxyphenylpyruvate dioxygenase (HPPD), the rate\u2010limiting step of tyrosine degradation, results in the death of insects after a blood meal. Therefore, it has been suggested that compounds that block the catabolism of tyrosine could act selectively on blood\u2010feeding insects. Here, we evaluated the toxicity against mosquitoes of three HPPD inhibitors currently used as herbicides and in human health.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Of the compounds tested, nitisinone (NTBC) proved to be more potent than mesotrione (MES) and isoxaflutole (IFT) in\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      . NTBC was lethal to\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      in artificial feeding assays [median lethal dose (LD\n                      <jats:sub>50<\/jats:sub>\n                      ): 4.53\u2009\u03bc\n                      <jats:sc>m<\/jats:sc>\n                      ] and in topical application (LD\n                      <jats:sub>50<\/jats:sub>\n                      : 0.012\u2009nmol\/mosquito). NTBC was also lethal to\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      populations that were resistant to neurotoxic insecticides, and to other mosquito species (\n                      <jats:italic>Anopheles<\/jats:italic>\n                      and\n                      <jats:italic>Culex<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>HPPD inhibitors, particularly NTBC, represent promising new drugs for mosquito control. Because they affect only blood\u2010feeding organisms, they represent a safer and more environmentally friendly alternative to conventional neurotoxic insecticides. \u00a9 2021 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.6679","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T02:56:21Z","timestamp":1634180181000},"page":"692-702","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["On the use of inhibitors of 4\u2010hydroxyphenylpyruvate dioxygenase as a vector\u2010selective insecticide in the control of mosquitoes"],"prefix":"10.1002","volume":"78","author":[{"given":"Marlon A","family":"Vergaray Ramirez","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Bioqu\u00edmica de Artr\u00f3podes Hemat\u00f3fagos, Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2266-9616","authenticated-orcid":false,"given":"Marcos","family":"Sterkel","sequence":"additional","affiliation":[{"name":"Centro Regional de Estudios Gen\u00f3micos Universidad Nacional de La Plata (CREG\u2010UNLP)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ademir J","family":"Martins","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiologia e Controle de Artr\u00f3podes Vetores, Instituto Oswaldo Cruz FIOCRUZ  Rio de Janeiro Brazil"},{"name":"Laborat\u00f3rio de Entomologia Instituto de Biologia do Ex\u00e9rcito  Rio de Janeiro Brazil"},{"name":"Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular (INCT\u2010EM)  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"BP Lima","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiologia e Controle de Artr\u00f3podes Vetores, Instituto Oswaldo Cruz FIOCRUZ  Rio de Janeiro Brazil"},{"name":"Laborat\u00f3rio de Entomologia Instituto de Biologia do Ex\u00e9rcito  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"L Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bioqu\u00edmica de Artr\u00f3podes Hemat\u00f3fagos, Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"},{"name":"Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular (INCT\u2010EM)  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5491.428"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15535"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010814-020828"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004006"},{"key":"e_1_2_8_6_1","volume-title":"Test Procedures for Insecticide Resistance Monitoring in Malaria Vector Mosquitoes","author":"WHO","year":"2018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2741\/2758"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005625"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2015.11.010"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.15272"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(16)00084-X"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.04.008"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.c.30092"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002594"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.06.025"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000796"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.03.015"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-59416-8_10"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1564\/20feb09"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-4173-4_7"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-4998(200102)57:2<120::AID-PS254>3.0.CO;2-E"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92685-9"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2004.12.017"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2011.04.016"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-4998(200102)57:2<133::AID-PS276>3.0.CO;2-0"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060878"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2410819"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1603\/ME10152"},{"key":"e_1_2_8_29_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0173689","article-title":"Toxicity of spinosad to temephos\u2010resistant Aedes aegypti populations in Brazil","volume":"12","author":"Santos DL","year":"2017","journal-title":"PLoS One"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173689"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","unstructured":"CostaM de M Salgueiro P Restrepo\u2010Zabaleta J Costa M Galardo AKR Pinto J et al. Liaisons dangereuses: cross\u2010border gene flow and dispersal of insecticide resistance\u2010associated genes in the mosquito Aedes aegypti from Brazil and French Guiana Memorias do Instituto Oswaldo Cruz114:e190120(2019).","DOI":"10.1590\/0074-02760190120"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.001589"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760140266"},{"key":"e_1_2_8_34_1","first-page":"407","article-title":"Studies on the feeding response of mosquitoes to nutritive solutions in a new membrane feeder","volume":"24","author":"Rutledge L","year":"1964","journal-title":"Mosq News"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.05.012"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1897\/05-320R.1"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1995.1121"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi000135h"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005799"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-216"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007485300041304"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2000.62.535"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000058"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-4571.2009.00072.x"},{"key":"e_1_2_8_45_1","article-title":"in vitro inhibition and induction of human liver cytochrome P450 enzymes by NTBC and its metabolism in human liver microsomes","volume":"42","author":"Neat JN","year":"2010","journal-title":"Drug Metab Rev"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180095"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5725"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4771"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.87.13"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-1825-9"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.19-0889"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu797"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-2125-0"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-1923-8"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.06.001"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1086\/653208"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-1801-4"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.05.007"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.43.229"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1603\/ME11164"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04512-5"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-007-9000-9"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1801338115"},{"key":"e_1_2_8_64_1","unstructured":"European Medicines Agency\u2010Orfadin INN\u2010Nitisinone 1\u2013102(2004)."},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0306-5251.2001.01421.x"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.6679","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.6679","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.6679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:47:02Z","timestamp":1761058022000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.6679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,27]]},"references-count":64,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/ps.6679"],"URL":"https:\/\/doi.org\/10.1002\/ps.6679","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/669747","asserted-by":"object"}]},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,27]]},"assertion":[{"value":"2021-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}