{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:50:01Z","timestamp":1790913001086,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21977083"],"award-info":[{"award-number":["21977083"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011195","name":"Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011195","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Powdery mildew is one of the fungal diseases commonly occurring in the process of cucurbits protected and open cultivation. Cucumbers, melons and pumpkins are extremely susceptible. The secondary metabolites produced by plants are important sources of fungicides with low toxicity and environment\u2010friendly characteristics. The aim of this study was to reveal the main active ingredient in the crude extracts of <jats:italic>Disporopsis aspera<\/jats:italic> rhizomes that inhibit cucurbits powdery mildew and evaluate its activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In this study, the crude extracts of <jats:italic>Disporopsis aspera<\/jats:italic> rhizomes were found to exhibit excellent antifungal activity aganist <jats:italic>Podosphaera xanthii<\/jats:italic>, a causal agent of cucurbits powdery mildew. Based on the bioassay\u2010guided method, <jats:sc>l<\/jats:sc>\u2010azetidine\u20102\u2010carboxylic acid (<jats:sc>l\u2010<\/jats:sc>Aze) was isolated from this genus for the first time. <jats:sc>l<\/jats:sc>\u2010Aze showed unique curative and eradicative activity against <jats:italic>Podosphaera xanthii in vivo<\/jats:italic>, which has never been reported before. Microscopic observation revealed that the curative spraying of <jats:sc>l<\/jats:sc>\u2010Aze could effectively inhibit the mycelial growth, resulting in hollow parts of the mycelia, not forming conidiophores, and interrupting the life cycle of powdery mildew. The eradicative spraying of <jats:sc>l<\/jats:sc>\u2010Aze caused the fracture of mycelia and deformity of conidiophores, which could not continue to produce conidia.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:sc>l<\/jats:sc>\u2010Aze was the main active ingredient of <jats:italic>D. aspera<\/jats:italic> against <jats:italic>Podosphaera xanthii<\/jats:italic>, which had both curative and eradicative effects. The results provided a strong possibility of using the crude extracts of <jats:italic>D. aspera<\/jats:italic> rhizomes and its main effective component, <jats:sc>l<\/jats:sc>\u2010Aze as biocontrol agents to control cucurbits powdery mildew.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6812","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T13:08:18Z","timestamp":1643288898000},"page":"1946-1952","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Antifungal activity of <scp>l<\/scp>\u2010azetidine\u20102\u2010carboxylic acid isolated from <i>Disporopsis aspera<\/i> rhizomes against <i>Podosphaera xanthii<\/i>"],"prefix":"10.1002","volume":"78","author":[{"given":"Lijuan","family":"Han","sequence":"first","affiliation":[{"name":"College of Chemistry &amp; Pharmacy, State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&amp;F University  Yangling P. R. China"},{"name":"Key Laboratory of Botanical Pesticide R&amp;D in Shaanxi Province  Yangling P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Chemistry &amp; Pharmacy, State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&amp;F University  Yangling P. R. China"},{"name":"Key Laboratory of Botanical Pesticide R&amp;D in Shaanxi Province  Yangling P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry &amp; Pharmacy, State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&amp;F University  Yangling P. R. China"},{"name":"Key Laboratory of Botanical Pesticide R&amp;D in Shaanxi Province  Yangling P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Chemistry &amp; Pharmacy, State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&amp;F University  Yangling P. R. China"},{"name":"Key Laboratory of Botanical Pesticide R&amp;D in Shaanxi Province  Yangling P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6613-4077","authenticated-orcid":false,"given":"Jiwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Chemistry &amp; Pharmacy, State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&amp;F University  Yangling P. R. China"},{"name":"Key Laboratory of Botanical Pesticide R&amp;D in Shaanxi Province  Yangling P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Botanical Pesticide R&amp;D in Shaanxi Province  Yangling P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(00)01683-6"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9468-7_62"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.00544"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-9326\/10\/9\/095004"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2008.00527.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.5423\/PPJ.RW.05.2012.0072"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811-020-02160-x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00351701"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0261-2194(00)00059-4"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1094\/phyto.2001.91.12.1166"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-020-02797-3"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-017-1049-8"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.05.061"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3744"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b05782"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4458"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015260931034"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201800090"},{"key":"e_1_2_9_20_1","first-page":"190","article-title":"Research on control methods of cucumber powdery mildew in greenhouse and lab","volume":"29","author":"Zhang X","year":"2005","journal-title":"J Hebei Normal Univ (Nat Sci Ed)"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1094\/phyto-71-596"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/b601097k"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo0515881"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/00304940609356436"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7ra08884a"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00246"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1094\/pd-80-1044"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/01140671.1994.9513818"},{"key":"e_1_2_9_29_1","first-page":"401","article-title":"Resistance of melon powdery mildew isolates to kresoxim\u2010methyl in Guangxi and determination of its sensitivity to three fungicides","volume":"45","author":"Lin S","year":"2014","journal-title":"J Southern Agric"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021763924798"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.6090\/jarq.44.217"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.6812","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.6812","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.6812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:19:34Z","timestamp":1761052774000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.6812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,10]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1002\/ps.6812"],"URL":"https:\/\/doi.org\/10.1002\/ps.6812","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,10]]},"assertion":[{"value":"2021-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}