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Although long\u2010term application of copper\u2010based fungicides is associated with ecotoxic effects on the environment, their use in viticulture is required until sustainable alternatives are available. <jats:italic>Trichoderma<\/jats:italic> spp. might be a promising approach to fungicide reduction while promoting plant growth and development and displaying biocontrol activity. This study aims to examine the tolerance and compatibility of <jats:italic>Trichoderma<\/jats:italic> spp. to copper fungicides. This work contributes to the development of a spray application consisting of a copper\u2010tolerant <jats:italic>Trichoderma<\/jats:italic> sp. combined with a downscaled copper fungicide rate against <jats:italic>P. viticola<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:italic>Trichoderma<\/jats:italic> spp. isolated from grapevine wood in vineyards were identified and used for tolerance screening in various concentrations of copper fungicides. Copper hydroxide was identified as being highly compatible with <jats:italic>Trichoderma<\/jats:italic>. Two <jats:italic>Trichoderma<\/jats:italic> candidates, <jats:italic>T. koningiopsis<\/jats:italic> and <jats:italic>T. harzianum<\/jats:italic>, showed high copper tolerance in mycelial growth and germination tests, and were adapted to 2.85\u2009g\u2009Cu\u2009L<jats:sup>\u22121<\/jats:sup> of the selected fungicide. Microscopic investigations showed the attachment of copper compounds to fungal cell walls and copper uptake within the cytoplasm. In the case of high tolerance, large\u2010scale copper uptake was prevented.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Our findings identified two highly copper\u2010tolerant <jats:italic>Trichoderma<\/jats:italic> isolates with natural adaptation to the vineyard ecosystem, which could be further tested as biostimulants and biocontrol agents, combined with a reduced fungicide rate for sustainable plant protection. \u00a9 2022 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.6951","type":"journal-article","created":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T14:18:32Z","timestamp":1651933112000},"page":"3266-3276","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<i>Trichoderma<\/i> species isolated from grapevine with tolerance towards common copper fungicides used in viticulture for plant protection"],"prefix":"10.1002","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8165-1507","authenticated-orcid":false,"given":"Verena","family":"K\u00fcpper","sequence":"first","affiliation":[{"name":"Institute for Plant Protection, Department of Phytomedicine State Education and Research Center of Viticulture, Horticulture and Rural Development (DLR) Rheinpfalz  Neustadt\/Weinstra\u00dfe Germany"},{"name":"Institute for Crop Science and Resource Conservation (INRES), Department of Plant Pathology University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Steiner","sequence":"additional","affiliation":[{"name":"Institute for Crop Science and Resource Conservation (INRES), Department of Plant Pathology University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Kortekamp","sequence":"additional","affiliation":[{"name":"Institute for Plant Protection, Department of Phytomedicine State Education and Research Center of Viticulture, Horticulture and Rural Development (DLR) Rheinpfalz  Neustadt\/Weinstra\u00dfe Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.geoderma.2007.05.007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2009.10.005"},{"key":"e_1_2_9_4_1","first-page":"17","volume-title":"Fachgespr\u00e4ch, Bedeutung von Kupfer f\u00fcr den Pflanzenschutz, insbesondere f\u00fcr den \u00f6kologischen Landbau \u2013 Reduktions\u2010 und Ersatzstrategien","author":"Berkelmann\u2010L\u00f6hnertz B","year":"2008"},{"key":"e_1_2_9_5_1","first-page":"16","article-title":"COMMISSION IMPLEMENTING REGULATION (EU) 2018\/1981 of 13 December 2018 renewing the approval of the active substances copper compounds, as candidates for substitution, in accordance 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