{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:52:27Z","timestamp":1786978347857,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005196","name":"Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["CAAS\u2010ZDRW202307"],"award-info":[{"award-number":["CAAS\u2010ZDRW202307"]}],"id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005196","name":"Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["YBXM2411"],"award-info":[{"award-number":["YBXM2411"]}],"id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Symbiotic microbes play a pivotal role in the feeding processes of phytophagous insects, and symbiosis has been established as a key strategy for certain species to acquire pectinases. However, whether symbiotic bacteria play a role in the pectinolytic metabolism of\n                      <jats:italic>Zeugodacus cucurbitae<\/jats:italic>\n                      remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Removal of symbiotic bacteria\n                      <jats:italic>via<\/jats:italic>\n                      egg sterilization significantly reduced larval food consumption, growth, and pectinase activity (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), highlighting that the microbiota was required for\n                      <jats:italic>Z. cucurbitae<\/jats:italic>\n                      larval growth under feeding on host plants. Microbial community analysis identified\n                      <jats:italic>Klebsiella spp.<\/jats:italic>\n                      as persistent colonizers of larval feeding wounds, exhibiting recycling between host tissues and plant substrates. Functional assays demonstrated that\n                      <jats:italic>Klebsiella<\/jats:italic>\n                      strains (CpL20, CpL49, CpL63, and CpL64) formed distinct hydrolysis zones on pectin medium and degraded pectin\n                      <jats:italic>via<\/jats:italic>\n                      high enzymatic activity (495.98\u2013830.54\u2009\u03bc\/mL). Reintroduction of\n                      <jats:italic>Klebsiella spp.<\/jats:italic>\n                      restored larval growth in sterile treatment groups, confirming their metabolic contribution.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Our results suggest that\n                      <jats:italic>Klebsiella spp.<\/jats:italic>\n                      circulate between the feeding environment and larval tissues, participating in the pectinolytic metabolism to utilize the host plant efficiently, thereby facilitating larval growth and development. This study provides a foundation for understanding the role of symbiotic bacteria in pectinolytic metabolism during the ecological adaptation of phytophagous insects and offers new insights into the environmentally friendly management of\n                      <jats:italic>Z. cucurbitae<\/jats:italic>\n                      in agricultural settings. \u00a9 2025 The Author(s).\n                      <jats:italic>Pest Management Science<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70035","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T05:11:33Z","timestamp":1751519493000},"page":"6820-6831","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in\n 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