{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:47:24Z","timestamp":1790948844153,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32220103009"],"award-info":[{"award-number":["32220103009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The sterile insect technique (SIT) represents a highly promising strategy for controlling medical and agricultural pests, and its success largely hinges on selecting an appropriate target gene. The Y chromosome, which contains crucial regulatory elements governing male sex determination and development, presents a potential source of such targets. However, its repetitive structures complicate the identification of Y\u2010chromosome genes. The chromosome quotient (CQ)\u2010based method has proven effective for identifying Y\u2010chromosome genes, including those with a high degree of repetition.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Five Y\u2010chromosome genes, including <jats:italic>Bdspermless<\/jats:italic>, were discovered in <jats:italic>Bactrocera dorsalis<\/jats:italic> (Diptera: Tephritidae) with the embryo male transcriptome as a reference. Interference with <jats:italic>Bdspermless<\/jats:italic> led to impaired testis development, reduced sperm count and viability, and a decline in offspring hatching rate.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The findings suggest that <jats:italic>Bdspermless<\/jats:italic> is a key gene for male reproduction in <jats:italic>B. dorsalis<\/jats:italic>, which can be a potential target for SIT. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.70051","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T15:30:59Z","timestamp":1752075059000},"page":"6991-6999","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification and functional analysis of the Y\u2010chromosome <scp><i>spermless<\/i><\/scp> in male reproduction of <scp><i>Bactrocera dorsalis<\/i><\/scp>"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8811-9898","authenticated-orcid":false,"given":"Li","family":"He","sequence":"first","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2013Australia Joint Research Center for Horticultural and Urban Pests Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University  Wuhan China"},{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenping","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2013Australia Joint Research Center for Horticultural and Urban Pests Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziniu","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2013Australia Joint Research Center for Horticultural and Urban Pests Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Qiao","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2013Australia Joint Research Center for Horticultural and Urban Pests Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, 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International Laboratory for Green Pest Control, Henan Engineering Laboratory of Pest Biological Control College of Plant Protection, Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6368-5903","authenticated-orcid":false,"given":"Hongyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2013Australia Joint Research Center for Horticultural and Urban Pests Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University  Wuhan 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