{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T17:19:47Z","timestamp":1778087987705,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22377045"],"award-info":[{"award-number":["22377045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22177043"],"award-info":[{"award-number":["22177043"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Xanthomonas oryzae<\/jats:italic> pv. <jats:italic>oryzae<\/jats:italic> (<jats:italic>Xoo<\/jats:italic>), the causal agent of rice bacterial blight, poses a significant threat to global crop security by means of systemic vascular colonization. In order to address emerging resistance challenges, a rational drug design strategy was employed, which involved structurally optimizing the natural alkaloid berberine (<jats:bold>BBR<\/jats:bold>). Through a C\u20109\/C\u201013 dual\u2010site modification approach, we developed a novel berberine derivative, 9\u2010benzoyloxy\u201013\u2010(4\u2010(tert\u2010butyl)benzyl)\u2010berberine bromide (<jats:bold>HBBR\u2010F4<\/jats:bold>). This compound demonstrated exceptional anti\u2010<jats:italic>Xoo<\/jats:italic> efficacy, achieving protective and curative effects of 62.57 and 65.23%, respectively, <jats:italic>in vivo<\/jats:italic>, outperforming the commercial bactericide thiodiazole copper.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:bold>HBBR\u2010F4<\/jats:bold> showed strong antibacterial activity against <jats:italic>Xoo<\/jats:italic> with the minimum inhibitory concentration and minimum bactericidal concentration values of 3.12 and 6.25\u2009\u03bcg\/mL, while showing negligible phytotoxicity. The results of transcriptomics showed that <jats:bold>HBBR\u2010F4<\/jats:bold> could inhibit the amination of cell membrane phospholipids, change the biosynthesis of membrane phospholipids, destroy the lipid balance of bacterial cell membrane, lead to cell membrane damage, intracellular nucleic acid leakage, SOS reaction and other bacterial dysfunction, thus inhibiting bacterial growth. This was confirmed by qRT\u2010PCR and related experiments. Observation of bacterial morphology showed that, following treatment with <jats:bold>HBBR\u2010F4<\/jats:bold>, <jats:italic>Xoo<\/jats:italic> cells exhibited irregular shapes, dissolved cytoplasms, broken cell membranes and vacuolated cytoplasms.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:bold>HBBR\u2010F4<\/jats:bold> alters the biosynthetic pathway of membrane phospholipids, disrupting the balance of bacterial cell membranes and causing functional damage to <jats:italic>Xoo<\/jats:italic>, making it a sustainable solution for the prevention and control of bacterial blight of rice. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.70064","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T05:19:05Z","timestamp":1753161545000},"page":"7140-7153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antibacterial activity and mechanism of novel berberine derivative <scp>HBBR<\/scp>\u2010<scp>F4<\/scp> against <scp><i>Xanthomonas oryzae<\/i><\/scp> pv. <scp><i>Oryzae<\/i><\/scp>"],"prefix":"10.1002","volume":"81","author":[{"given":"Liu","family":"Yang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo\u2010Liang","family":"Mou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7384-4763","authenticated-orcid":false,"given":"Hong\u2010Jie","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010Ya","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Lanzhou University  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