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Bioassay results manifested that compound\n                      <jats:bold>C<\/jats:bold>\n                      <jats:sub>\n                        <jats:bold>19<\/jats:bold>\n                      <\/jats:sub>\n                      exhibited remarkable antibacterial efficacy against\n                      <jats:italic>Xanthomonas oryzae<\/jats:italic>\n                      pv.\n                      <jats:italic>oryzae<\/jats:italic>\n                      (\n                      <jats:italic>Xoo<\/jats:italic>\n                      ) and\n                      <jats:italic>Xanthomonas axonopodis<\/jats:italic>\n                      pv.\n                      <jats:italic>citri<\/jats:italic>\n                      (\n                      <jats:italic>Xac<\/jats:italic>\n                      ), with EC\n                      <jats:sub>50<\/jats:sub>\n                      values of 2.16\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 4.64\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , respectively. Transcriptomic analysis initially revealed that compound\n                      <jats:bold>C<\/jats:bold>\n                      <jats:sub>\n                        <jats:bold>19<\/jats:bold>\n                      <\/jats:sub>\n                      involved significant downregulation of genes associated with biofilm, type III secretion system (T3SS), and flagella assembly. Subsequent multiple validations\n                      <jats:italic>via<\/jats:italic>\n                      biofilm formation assays, bacterial ultrastructure analysis, motility assays, virulence evaluation, and hypersensitive response (HR) assays, collectively indicated that compound\n                      <jats:bold>C<\/jats:bold>\n                      <jats:sub>\n                        <jats:bold>19<\/jats:bold>\n                      <\/jats:sub>\n                      both inhibited biofilm formation and suppressed swimming ability while reducing the expression of\n                      <jats:italic>hrp<\/jats:italic>\n                      family genes in T3SS, ultimately attenuating\n                      <jats:italic>Xoo<\/jats:italic>\n                      pathogenicity. Notably, Pot experiment demonstrated that compound\n                      <jats:bold>C<\/jats:bold>\n                      <jats:sub>\n                        <jats:bold>19<\/jats:bold>\n                      <\/jats:sub>\n                      exhibited superior control efficacy (protective activity: 42.74%; curative activity: 44.14%), surpassing commercial BT and TC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Given its excellent antibacterial potency and the mechanism of action elucidated through transcriptome analysis and ample biochemical assays, compound\n                      <jats:bold>C<\/jats:bold>\n                      <jats:sub>\n                        <jats:bold>19<\/jats:bold>\n                      <\/jats:sub>\n                      represented a promising candidate for developing novel bactericides\n                      <jats:italic>via<\/jats:italic>\n                      targeting bacterial virulence to combat refractory plant bacterial diseases. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70091","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T09:20:56Z","timestamp":1754299256000},"page":"7777-7792","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of imidazo[1,2\u2010\n                    <i>a<\/i>\n                    ]pyridine skeleton as new bactericidal candidates: structural innovation and virulence\u2010targeted behavior"],"prefix":"10.1002","volume":"81","author":[{"given":"Jun\u2010Rong","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Green Pesticides, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education 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