{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:09:27Z","timestamp":1789787367577,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T00:00:00Z","timestamp":1754697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The Gram\u2010negative bacterium\n                      <jats:italic>Erwinia amylovora<\/jats:italic>\n                      induces fire blight disease in Rosaceae hosts, severely damaging pome fruit trees including pear, apple, and hawthorn cultivars. Traditional control methods, such as antibiotics and copper\u2010based agents, are associated with plant damage and the emergence of resistant bacterial strains, and present significant challenges. This study aims to design and synthesize a novel eco\u2010friendly bactericide.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In this study, we designed and synthesized 30 small\u2010molecule compounds by combining 1,3,4\u2010thiadiazole, a key moiety of zinc thiazole, with 5\u2010phenyl\u20102\u2010furan. Structural characterization of these compounds was performed using\n                      <jats:sup>1<\/jats:sup>\n                      H and\n                      <jats:sup>13<\/jats:sup>\n                      C NMR spectroscopy, complemented by elemental composition analysis. Compound\n                      <jats:bold>II\u201010<\/jats:bold>\n                      was identified as the most promising compound in initial screenings, with a median effective concentration (EC\n                      <jats:sub>50<\/jats:sub>\n                      ) of 9.04\u2009\u03bcg\/mL. Inoculation experiments demonstrated that at a concentration of 200\u2009\u03bcg\/mL,\n                      <jats:bold>II\u201010<\/jats:bold>\n                      effectively inhibited\n                      <jats:italic>E. amylovora<\/jats:italic>\n                      and controlled pear fire blight, with its protective activity exceeding its curative effects. Furthermore, fluorescence microscopy, flow cytometry and scanning electron microscopy analysis revealed a potential mechanism of antimicrobial action involving reactive oxygen species generation, apoptosis and changes in bacterial morphology. Toxicity assessment showed that\n                      <jats:bold>II\u201010<\/jats:bold>\n                      was non\u2010toxic to zebrafish, earthworm and silkworm.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This study established that\n                      <jats:bold>II\u201010<\/jats:bold>\n                      was a lead scaffold for the development to control pear fire blight, providing theoretical support for optimizing small\u2010molecule compounds. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70104","type":"journal-article","created":{"date-parts":[[2025,8,10]],"date-time":"2025-08-10T04:49:40Z","timestamp":1754801380000},"page":"7928-7938","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inhibitory activity and mechanism of aryl furan thioesters containing 1,3,4\u2010thiadiazole against\n                    <i>Erwinia amylovora<\/i>"],"prefix":"10.1002","volume":"81","author":[{"given":"Junjie","family":"Wei","sequence":"first","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangye","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, 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