{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:02Z","timestamp":1786978382633,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Maternally inherited symbionts can impact the reproduction of their host insects in various ways. The ovary is critical for the successful reproduction of female insects. Such symbionts have strong ovary tropism. Intracellular symbionts depend on host cells for replication and transmission. However, the cellular mechanisms by which symbionts impact insect reproduction through affecting ovary development have seldom been investigated. Our previous work has shown that the facultative symbiont\n Rickettsia<\/jats:italic>\n can be vertically transmitted through eggs in the whitefly\n Bemisia tabaci<\/jats:italic>\n and\n Rickettsia<\/jats:italic>\n increases female fecundity. This study is aimed to explore the cellular mechanisms of\n Rickettsia<\/jats:italic>\n symbionts affecting the whitefly fecundity.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n We found that\n Rickettsia<\/jats:italic>\n symbionts are highly concentrated in the tropharium of whitefly ovarioles. We demonstrated that\n Rickettsia<\/jats:italic>\n increases whitefly fecundity by promoting ovary development. Moreover,\n Rickettsia<\/jats:italic>\n enhances the expression of cell division genes (\n Cyclin B1<\/jats:italic>\n and\n CDK1<\/jats:italic>\n ) and germ cell mitosis. Furthermore,\n Cyclin B1<\/jats:italic>\n and\n CDK1<\/jats:italic>\n gene silencing or CDK1 inhibitor treatment reduced the germ cell mitosis, delayed ovary development and decreased whitefly fecundity.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Overall, we revealed that\n Rickettsia<\/jats:italic>\n promotes ovary development by regulating germ cell mitosis, which enhances whitefly fecundity. These findings suggest that regulating the host cell cycle by the symbiont is important for the maintenance of the intracellular symbiosis. This study provides new insights into the cellular mechanism of symbionts regulating host reproduction. Our study also provides excellent genetic targets for insect control. \u00a9 2025 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.70167","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T08:01:28Z","timestamp":1756195288000},"page":"8624-8632","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["\n Rickettsia<\/i>\n <\/scp>\n symbionts favor whitefly ovary development by promoting germ cell mitosis"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2430-0230","authenticated-orcid":false,"given":"Nai\u2010Fei","family":"Chen","sequence":"first","affiliation":[{"name":"Shenyang Key Laboratory of Surveillance and Management for Vegetable Diseases and Insect Pests, College of Plant Protection Shenyang Agricultural University Shenyang China"},{"name":"Liaoning Key Laboratory of Economic and Applied Entomology, College of 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