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However, it remains unclear how the JH synthase\u2010encoding gene (\n                      <jats:italic>DcJHAMT<\/jats:italic>\n                      ) and the degradation enzyme\u2010encoding gene (\n                      <jats:italic>DcJHEH<\/jats:italic>\n                      ) are involved in regulating JH levels, vitellogenin synthesis and ovarian development.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      This study employed RNA interference (RNAi) to silence\n                      <jats:italic>DcJHAMT<\/jats:italic>\n                      and\n                      <jats:italic>DcJHEH<\/jats:italic>\n                      in female\n                      <jats:italic>D.\u2009citri<\/jats:italic>\n                      . After\n                      <jats:italic>DcJHAMT<\/jats:italic>\n                      was silenced, the JH concentration decreased, the expression levels of key vitellogenin genes (\n                      <jats:italic>Vg<\/jats:italic>\n                      and\n                      <jats:italic>VgR<\/jats:italic>\n                      ) decreased, and ovarian development slowed. After\n                      <jats:italic>DcJHEH<\/jats:italic>\n                      was silenced, the JH concentration increased, the expression levels of\n                      <jats:italic>Vg<\/jats:italic>\n                      and\n                      <jats:italic>VgR<\/jats:italic>\n                      were upregulated, and ovarian development accelerated. After interfering with\n                      <jats:italic>DcJHAMT<\/jats:italic>\n                      , the expression level of\n                      <jats:italic>DcJHEH<\/jats:italic>\n                      decreased, while after interfering with\n                      <jats:italic>DcJHEH<\/jats:italic>\n                      , the expression level of\n                      <jats:italic>DcJHAMT<\/jats:italic>\n                      increased, which confirms that the\n                      <jats:italic>DcJHAMT<\/jats:italic>\n                      and\n                      <jats:italic>DcJHEH<\/jats:italic>\n                      genes are antagonistic to each other and jointly regulate the dynamic balance of JH.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      <jats:italic>DcJHAMT<\/jats:italic>\n                      and\n                      <jats:italic>DcJHEH<\/jats:italic>\n                      were found to synergistically regulate the JH titer and vitellogenin production of\n                      <jats:italic>D.\u2009citri<\/jats:italic>\n                      . These results will contribute to the in\u2010depth study of JH pathway regulation in\n                      <jats:italic>D.\u2009citri<\/jats:italic>\n                      and bestow significant data for the development of RNAi green pesticides based on reproductive regulatory genes. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70257","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T08:51:02Z","timestamp":1758876662000},"page":"992-1003","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Regulation of reproduction by juvenile hormone synthase and degradation enzyme in\n                    <scp>\n                      <i>Diaphorina citri<\/i>\n                    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