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R. glutinosa<\/jats:italic>\n                      (FORg), a pathogenic strain isolated from\n                      <jats:italic>R. glutinosa<\/jats:italic>\n                      , induces root rot disease, leading to severe yield losses. Currently, resistant cultivars and effective control strategies for\n                      <jats:italic>R. glutinosa<\/jats:italic>\n                      root rot remain very limited. This study aims to investigate the feasibility and potential of SIGS technology in preventing and controlling\n                      <jats:italic>R. glutinosa<\/jats:italic>\n                      root rot caused by FORg.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      This study demonstrates that FORg hyphae can capably take up dsRNA, confirming their suitability for SIGS\u2010based disease control. dsRNA targeting\n                      <jats:italic>SGE1<\/jats:italic>\n                      and\n                      <jats:italic>CYP51,<\/jats:italic>\n                      synthesized\n                      <jats:italic>in vivo<\/jats:italic>\n                      and extracted through low\u2010cost ethanol method, significantly suppressed the expression of virulence\u2010associated genes in both hyphae and conidia. Root disc assays further validated that exogenous dsRNA effectively attenuated FORg pathogenicity. Combined applications of\n                      <jats:italic>SGE1<\/jats:italic>\n                      and\n                      <jats:italic>CYP51S<\/jats:italic>\n                      dsRNAs synergistically reduced disease severity compared to single\u2010target treatments. Additionally, dsRNA encapsulated in layered double hydroxide (LDH) prolonged functional durability, with sustained plant protection observed for 15\u2009days post\u2010spraying, outperforming unencapsulated dsRNA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This finding establishes the feasibility of SIGS for controlling FORg\u2010induced root rot in\n                      <jats:italic>R. glutinosa<\/jats:italic>\n                      and highlights the potential of nanomaterial encapsulation in addressing persistent fungal epidemics in agricultural systems. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  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