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The fall webworm,\n                      <jats:italic>Hyphantria cunea<\/jats:italic>\n                      , is a highly polyphagous lepidopteran pest that poses significant threats to forestry and agriculture because of its broad host range and high reproductive potential. Despite studies on its feeding ecology, the molecular mechanisms underlying its aversive response to bitter compounds remain largely unexplored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      We demonstrate that quinine significantly suppresses feeding in\n                      <jats:italic>H. cunea<\/jats:italic>\n                      larvae. Using calcium imaging and intracellular calcium concentration measurements via a microplate reader, we identified a bitter gustatory receptor, HcGr148, that specifically responds to quinine. Quantitative real\u2010time polymerase chain reaction analysis revealed that\n                      <jats:italic>HcGr148<\/jats:italic>\n                      is highly expressed in the mouthpart sensory organs and brain. Moreover, RNA interference via injection of double\u2010stranded RNA targeting HcGr148 markedly reduced the sensitivity of the maxillary taste sensilla to quinine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Our findings reveal key components of the bitter taste signaling in\n                      <jats:italic>H. cunea<\/jats:italic>\n                      and shed light on how this pest species discriminates against unpalatable food sources at the molecular level, thereby offering useful insights into bitter taste perception in lepidopterans and suggesting possible avenues for future pest management research. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  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