{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T14:05:44Z","timestamp":1784642744041,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      With the development of rapid resistance, new modes of action for pesticides are needed for insect control, such as RNA interference (RNAi)\u2010based biopesticides targeting essential genes. To explore the function of Argonaute\u20101 (Ago\u20101) and potential microRNAs (miRNAs) in ovarian development and their impact on rapid resistance in important storage pest\n                      <jats:italic>Tribolium castaneum<\/jats:italic>\n                      , we knocked down\n                      <jats:italic>Ago\u20101<\/jats:italic>\n                      and observed the development of ovarian and insecticide susceptibility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Our results indicated that\n                      <jats:italic>Ago\u20101<\/jats:italic>\n                      had a relatively high transcriptional level of adult female\n                      <jats:italic>T. castaneum<\/jats:italic>\n                      during the sexual maturation period. RNAi experiment showed that\n                      <jats:italic>Ago\u20101<\/jats:italic>\n                      knockdown significantly decreased the expression levels of ovarian development\u2010related genes and disrupted ovarian development and the morphology was atrophied. Reproductive experiments showed that the egg laying rate of female beetles was close to zero, while the fertility of male beetles was not affected. The lipid droplet of the beetles in\n                      <jats:italic>dsAgo\u20101<\/jats:italic>\n                      group was significantly higher than\n                      <jats:italic>dsEGFP<\/jats:italic>\n                      group, which may be due to ovarian abnormalities caused by nutrient deficiency. The quantitative polymerase chain reaction (qPCR) results showed that the expression level of\n                      <jats:italic>Ago\u20101<\/jats:italic>\n                      significantly increased after different pesticide treatments, and knocking down significantly increased the mortality rate of beetles treated with pesticides.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      The results indicate that\n                      <jats:italic>Ago\u20101<\/jats:italic>\n                      is indispensable for normal ovarian development in\n                      <jats:italic>T. castaneum<\/jats:italic>\n                      and helps to address the issue of resistance development by enhancing insecticide susceptibility. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70299","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T10:32:16Z","timestamp":1760610736000},"page":"1492-1499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>RNA<\/scp>\n                    interference of\n                    <scp>\n                      <i>Argonaute\u20101<\/i>\n                    <\/scp>\n                    disrupts ovarian development and enhances insecticide susceptibility in\n                    <scp>\n                      <i>Tribolium castaneum<\/i>\n                    <\/scp>"],"prefix":"10.1002","volume":"82","author":[{"given":"Peng","family":"chen","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiping","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Fang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4544-4532","authenticated-orcid":false,"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-031616-034925"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.10.006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600385"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.09.034"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1972910"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.10.009"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26129-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7041"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2020.104700"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006034"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.170670"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00301"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6419"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1609792113"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/tox122"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3395"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toaa120"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6306"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.51.110104.151104"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2015.01.006"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2018.04.006"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072449"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2008.09.002"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.08.162"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2015.11.001"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3340"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6419"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-1-r10"},{"key":"e_1_2_9_31_1","first-page":"1","article-title":"Phenolic profile and biopesticidal potential of Moringa oleifera extracts: antioxidant and insecticidal activities against stored pests","volume":"0","author":"Bachrouch O","year":"2025","journal-title":"Int J Environ Health Res"},{"key":"e_1_2_9_32_1","doi-asserted-by":"crossref","first-page":"3554","DOI":"10.2174\/1381612826666200513113140","article-title":"Tribolium castaneum: a model for investigating the mode of action of insecticides and mechanisms of resistance","volume":"26","author":"R\u00f6sner J","year":"2024","journal-title":"Curr Pharm Des"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12327"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2009.08.005"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2017.06.001"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.03.005"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8158"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7917.2012.01555.x"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2010.04.004"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.04.002"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/B0-44-451924-6\/00040-5"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8076"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21688"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232213832"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00946"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1359"},{"key":"e_1_2_9_48_1","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.bbagrm.2018.04.001","article-title":"Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies","volume":"1861","author":"Rubio M","year":"2018","journal-title":"Biochim Biophys Acta (BBA)"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12848"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.70069"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4492"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105347"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202308028"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.974646"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12668-022-01003-y"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.70299","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T09:00:36Z","timestamp":1768122036000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.70299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,16]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/ps.70299"],"URL":"https:\/\/doi.org\/10.1002\/ps.70299","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,16]]},"assertion":[{"value":"2025-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}