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We previously identified in a high\u2010throughput screen six small molecules SACC\u20100412060, SACC\u20100412062, SACC\u20100412066, SACC\u20100064443, SACC\u20100428788, and SACC\u20100039590 as antagonists of the kinin receptor from\n                      <jats:italic>Rhipicephalus microplus<\/jats:italic>\n                      . The acaricidal activity of these molecules was investigated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The toxicities of adjuvant\/solvent combinations of a rapeseed oil methyl ester or a propylene carbonate in 5% DMSO\n                      <jats:italic>versus<\/jats:italic>\n                      5% DMSO alone were evaluated using a larval immersion test (LIT) with the\n                      <jats:italic>R. microplus<\/jats:italic>\n                      susceptible Deutch strain, and all were non\u2010toxic. The rapeseed oil methyl ester (1%) in 5% DMSO was adopted as the adjuvant for all molecules in bioassays. Permethrin (3.19\u2009mM) killed all larvae. The Deutch strain mortality with SACC\u20100039590 and SACC\u20100428788 (both at 1\u2009mM) was 100% and 73.65%, respectively, while ranged from 2.7% to 3.8% with the other small molecules. In the LIT, the LC\n                      <jats:sub>50<\/jats:sub>\n                      of SACC\u20100039590 and SACC\u20100428788 were 60\u2009\u03bcM, and 450\u2009\u03bcM, respectively. For the\n                      <jats:italic>R. microplus<\/jats:italic>\n                      Arauquita pyrethroid\u2010resistant strain in the LIT, the LC\n                      <jats:sub>50<\/jats:sub>\n                      of SACC\u20100039590 and SACC\u20100428788 were 60\u2009\u03bcM and 237.5\u2009\u03bcM respectively. SACC\u20100039590 (N\u2010[2\u2010(4\u2010chlorophenyl)ethyl]\u20105\u2010methylthieno[2,3\u2010d]pyrimidin\u20104\u2010amine) and SACC\u20100428788 (N\u2010[2\u2010(4\u2010chlorophenyl)ethyl]thieno[2,3\u2010d]pyrimidin\u20104\u2010amine) are structural analogs, the first one featuring a 5\u2010methyl group absent in the second. For engorged Deutch females, immersion in SACC\u20100039590 caused dose\u2010dependent reductions in egg mass weight (~35%) and egg hatching (21%\u201338.59%). Permethrin (3.19\u2009mM) significantly reduced egg mass weight but not egg hatching.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      The small molecule SACC\u20100039590 killed susceptible and pyrethroid\u2010resistant larvae of\n                      <jats:italic>R. microplus<\/jats:italic>\n                      and reduced the reproductive output of females. \u00a9 2025 The Author(s).\n                      <jats:italic>Pest Management Science<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  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