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Endophytes are well\u2010known producers of bioactive secondary metabolites, offering potential for environmentally friendly control agents. This study focused on identifying and evaluating nematicidal compounds from pine tree endophytes active against PWN.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      A total of 30 endophytes isolated from Korean pine trees were screened for their nematicidal activity and identified as novel nematicidal agents against PWN. Among them,\n                      <jats:italic>Streptomyces sp.<\/jats:italic>\n                      AN140557 showed the highest nematicidal activity. The active compound, murayaquinone, was isolated by nematicidal activity\u2010guided fractionation, and it showed significant dose\u2010dependent nematicidal activity and egg\u2010hatching inhibition against PWN at 3.12, 6.25, 12.5, and 25\u2009\u03bcM, respectively. Exposure to 25\u2009\u03bcM murayaquinone led to 100% mortality of PWN. Additionally, murayaquinone strongly suppressed egg hatching. In addition, the assessment of the activity against the other four plant parasitic nematodes (\n                      <jats:italic>Meloidogyne incognita<\/jats:italic>\n                      ,\n                      <jats:italic>Ditylenchus destructor<\/jats:italic>\n                      ,\n                      <jats:italic>Aphelenchoides subtenuis<\/jats:italic>\n                      , and\n                      <jats:italic>Heterodera trifolii<\/jats:italic>\n                      ) showed that it has a broad nematicidal spectrum. The greenhouse experiments suggested that the murayaquinone efficiently inhibited the development of PWD in 5\u2010year\u2010old\n                      <jats:italic>Pinus thunbergii<\/jats:italic>\n                      plants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Our results highlight the nematicidal potential of the murayaquinone derived from\n                      <jats:italic>Streptomyces sp.<\/jats:italic>\n                      AN140557. This is the first report of murayaquinone exhibiting biocontrol potential against PWN, suggesting its possibility as a PWD control agent. \u00a9 2025 The Author(s).\n                      <jats:italic>Pest Management Science<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70324","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:42:08Z","timestamp":1761720128000},"page":"1765-1775","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discovery of murayaquinone from an endophytic bacterium\n                    <i>Streptomyces sp.<\/i>\n                    <scp>AN140557<\/scp>\n                    as a potent inhibitor of the pine wood nematode,\n                    <i>Bursaphelenchus xylophilus<\/i>"],"prefix":"10.1002","volume":"82","author":[{"given":"Min\u2010Kyoung","family":"Kang","sequence":"first","affiliation":[{"name":"Sensor System Research Center Korea Institute of Science and Technology (KIST)  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeon\u2010Ji","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Pukyong National University  Busan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4777-8007","authenticated-orcid":false,"given":"Jong\u2010Hoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Pukyong National University  Busan Republic of Korea"},{"name":"Microbiome Convergence Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","article-title":"Investigation on pine mortality in Nagasaki prefecture","volume":"1","author":"Yano H","year":"1913","journal-title":"J Jpn For Soc"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.21.090183.001221"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/f14020174"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.13875"},{"key":"e_1_2_9_6_1","first-page":"1","article-title":"Pine wilt disease in Japan: from first occurrence to the present","volume":"39","author":"Mamiya Y","year":"2009","journal-title":"Jpn J Nematol"},{"key":"e_1_2_9_7_1","first-page":"307","article-title":"Emergence ecology of Japanese pine sawyer, Monochamus alternatus (coleoptera: Cerambycidae), a vector of pinewood nematode, Bursaphelenchus xylophilus","volume":"42","author":"Kim DS","year":"2003","journal-title":"Korean J Appl Entomol"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10144-006-0266-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-081211-172910"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/IECF2020-08003"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13202876"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2np00074a"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12600-013-0344-y"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2022.2044350"},{"key":"e_1_2_9_15_1","unstructured":"U.S. Environmental Protection Agency Preliminary Ecological Risk Assessment for Abamectin. 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