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However, its functional significance in insects remains poorly characterized. In this study, we cloned\n                    <jats:italic>TRPML<\/jats:italic>\n                    of\n                    <jats:italic>Nilaparvata lugens<\/jats:italic>\n                    (\n                    <jats:italic>NlTRPML<\/jats:italic>\n                    ), employed bioinformatics tools to predict its structure and function, elucidating its tertiary architecture and potential binding sites for the small\u2010molecule agonist mucolipin synthetic agonist 1(ML\u2010SA1). Quantitative real\u2010time (qRT)\u2010PCR results showed that\n                    <jats:italic>NlTRPML<\/jats:italic>\n                    was ubiquitously expressed across all developmental stages and tissues of\n                    <jats:italic>N.\u2009lugens<\/jats:italic>\n                    , and especially highly in the ovaries of pregnant females\n                    <jats:italic>.<\/jats:italic>\n                    Immunofluorescence analysis further revealed that\n                    <jats:italic>Nl<\/jats:italic>\n                    TRPML was widely present in the female internal reproductive system. Interestingly,\n                    <jats:italic>NlTRPML<\/jats:italic>\n                    was found highly expressed in epithelial plug of ovarioles when yeast\u2010like symbionts (YLSs) entered it. RNA interference (RNAi) of\n                    <jats:italic>NlTRPML<\/jats:italic>\n                    expression resulted in delayed ovarian development, blocked ovulation, and reduced translocation of YLSs into oocytes. Conversely, ML\u2010SA1 injection markedly increased the number of YLSs in oocytes. These findings demonstrate that\n                    <jats:italic>Nl<\/jats:italic>\n                    TRPML is indispensable to the reproduction of\n                    <jats:italic>N.\u2009lugens<\/jats:italic>\n                    and the transovarial transmission of YLSs. \u00a9 2025 Society of Chemical Industry.\n                  <\/jats:p>","DOI":"10.1002\/ps.70336","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T20:17:16Z","timestamp":1762201036000},"page":"1944-1957","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The calcium ion channel protein\n                    <scp>TRPML<\/scp>\n                    is indispensable to the reproduction of\n                    <scp>\n                      <i>Nilaparvata lugens<\/i>\n                    <\/scp>\n                    and the transovarial transmission of its yeast\u2010like 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