{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T03:12:39Z","timestamp":1785208359062,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160637"],"award-info":[{"award-number":["32160637"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n The reproductive plasticity of female insects is governed by the complex structure and function of their ovaries. However, the molecular mechanisms underlying ovarian development in\n Lasioderma serricorne<\/jats:italic>\n , a globally significant pest of stored products, remain largely unexplored. The role of microRNAs (miRNAs) in regulating genes associated with reproduction remains poorly characterized.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n This study investigated the regulatory mechanisms involving the\n LsHSP70\u20103<\/jats:italic>\n gene, which encodes a heat shock protein, and its associated miRNA in the reproductive processes of female\n L. serricorne<\/jats:italic>\n . RNA interference\u2010mediated knockdown of\n LsHSP70\u20103<\/jats:italic>\n markedly impaired ovarian development and reduced female fecundity.\n In silico<\/jats:italic>\n predictions, validated by quantitative real\u2010time polymerase chain reaction, identified\n Lse\u2010miR\u20102944c\u20103p<\/jats:italic>\n as the cognate miRNA targeting\n LsHSP70\u20103<\/jats:italic>\n . Dual\u2010luciferase reporter assays and RNA pull\u2010down experiments confirmed the interaction between\n Lse\u2010miR\u20102944c\u20103p<\/jats:italic>\n and the 3\u2032 untranslated region of\n LsHSP70\u20103<\/jats:italic>\n , resulting in the repression of its expression. Microinjection of\n Lse\u2010miR\u20102944c\u20103p<\/jats:italic>\n mimics led to reduced expression of\n LsHSP70\u20103<\/jats:italic>\n and down\u2010regulation of vitellogenin (\n Vg<\/jats:italic>\n ) and\n Vg<\/jats:italic>\n receptor (\n VgR<\/jats:italic>\n ) transcripts, culminating in impaired ovarian development, decreased oviposition, and reduced egg hatchability. Conversely, administration of an\n Lse\u2010miR\u20102944c\u20103p<\/jats:italic>\n inhibitor elevated the expression of\n LsHSP70\u20103<\/jats:italic>\n ,\n LsVg<\/jats:italic>\n , and\n LsVgR<\/jats:italic>\n , thereby promoting oogenesis and increasing egg production. Rescue experiments involving the co\u2010injection of the miRNA inhibitor and ds\n LsHSP70\u20103<\/jats:italic>\n corroborated that\n Lse\u2010miR\u20102944c\u20103p<\/jats:italic>\n modulates reproduction by fine\u2010tuning\n LsHSP70\u20103<\/jats:italic>\n expression.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n The findings suggested that\n LsHSP70\u20103<\/jats:italic>\n deficiency impairs reproductive development in\n L<\/jats:italic>\n .\n serricorne<\/jats:italic>\n females.\n Lse\u2010miR\u20102944c\u20103p<\/jats:italic>\n and its target,\n LsHSP70\u20103<\/jats:italic>\n , represent potential molecular targets for the control of\n L. serricorne<\/jats:italic>\n . \u00a9 2025 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.70352","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T05:41:56Z","timestamp":1762494116000},"page":"2099-2109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["\n MicroRNA\n Lse\u2010miR\u20102944c\u20103p<\/i>\n <\/scp>\n regulates female reproduction in\n \n Lasioderma serricorne<\/i>\n <\/scp>\n by targeting the heat shock protein\n \n LsHSP70\u20103<\/i>\n <\/scp>"],"prefix":"10.1002","volume":"82","author":[{"given":"Kang\u2010Kang","family":"Xu","sequence":"first","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Province, College of Biological and Environmental Engineering Guiyang University Guiyang 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