{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T03:15:06Z","timestamp":1781666106615,"version":"3.54.5"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T00:00:00Z","timestamp":1762819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Despite the revelation of numerous uncharacterized biosynthetic gene clusters (BGCs) in the termite\u2010associated fungus\n                      <jats:italic>Aspergillus chevalieri<\/jats:italic>\n                      BYST01 through genome mining, no suitable culture conditions have been established to activate the production of new secondary metabolites.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Herein, using the OSMAC (one strain many compounds) strategy to preliminarily analyze the potential metabolites of\n                      <jats:italic>A. chevalieri<\/jats:italic>\n                      BYST01. A total of 23 metabolites were isolated and identified from minimal medium cultures in\n                      <jats:italic>A. chevalieri<\/jats:italic>\n                      BYST01, including one new compound (\n                      <jats:bold>2<\/jats:bold>\n                      ) and 22 known compounds (\n                      <jats:bold>1<\/jats:bold>\n                      ,\n                      <jats:bold>3<\/jats:bold>\n                      \u2013\n                      <jats:bold>23<\/jats:bold>\n                      ), which were categorized into seven distinct skeletal types. Partial metabolites were evaluated for their antibacterial and phytotoxic activities. Pannorin C (\n                      <jats:bold>1<\/jats:bold>\n                      ) exhibited strong antibacterial activities against\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      ,\n                      <jats:italic>Pseudomonas syringae<\/jats:italic>\n                      pv.\n                      <jats:italic>actinidae<\/jats:italic>\n                      ,\n                      <jats:italic>Xanthomonas oryzae<\/jats:italic>\n                      pv.\n                      <jats:italic>oryzae<\/jats:italic>\n                      (Xoo) and\n                      <jats:italic>X. oryzae<\/jats:italic>\n                      pv.\n                      <jats:italic>oryzicola<\/jats:italic>\n                      (Xoc). Citreorosein (\n                      <jats:bold>18<\/jats:bold>\n                      ) and stemphyperylenol (\n                      <jats:bold>19<\/jats:bold>\n                      ) showed excellent antibacterial activity against Xoc with inhibition zone diameters (IZD) of 15.5 and 19.6\u2009mm, respectively. Notably, dihydroauroglaucin (\n                      <jats:bold>6<\/jats:bold>\n                      ), flavoglaucin (\n                      <jats:bold>9<\/jats:bold>\n                      ), and compound\n                      <jats:bold>18<\/jats:bold>\n                      demonstrated significant protective efficacy against rice bacterial blight at 0.5\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , with protection rates of 84.82, 82.68, and 82.88%, respectively, which was stronger than that of commercial bactericide kasugamycin (81.52%). Additionally, compound\n                      <jats:bold>19<\/jats:bold>\n                      and vivotoxin II (\n                      <jats:bold>23<\/jats:bold>\n                      ) exhibited strong inhibitory effects against the root growth of the\n                      <jats:italic>Echinochloa crusgalli<\/jats:italic>\n                      with an inhibition rate of 67.6 and 87.6% at the concentration of 100\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , respectively. Tenuazonic acid (\n                      <jats:bold>22<\/jats:bold>\n                      ) and vivotoxin II (\n                      <jats:bold>23<\/jats:bold>\n                      ) significantly inhibited the root growth of\n                      <jats:italic>Abutilon theophrasti<\/jats:italic>\n                      at the concentration of 100\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      (82.5 and 100%, respectively). The possible herbicidal mechanisms of compounds\n                      <jats:bold>19<\/jats:bold>\n                      ,\n                      <jats:bold>22<\/jats:bold>\n                      , and\n                      <jats:bold>23<\/jats:bold>\n                      were preliminarily investigated by molecular docking simulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>These findings validate OSMAC for uncovering bioactive metabolites from BYST01 and provide lead compounds for the development of bioactive drugs in food and agricultural fields. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70356","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T07:00:31Z","timestamp":1762930831000},"page":"2132-2141","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The\n                    <scp>OSMAC<\/scp>\n                    strategy unveils new naphthopyranon and diverse bioactive metabolites from termite\u2010associated\n                    <scp>\n                      <i>Aspergillus chevalieri<\/i>\n                      BYST01\n                    <\/scp>"],"prefix":"10.1002","volume":"82","author":[{"given":"Zhong\u2010di","family":"Huang","sequence":"first","affiliation":[{"name":"School of Life Sciences, Joint Research Center for Food Nutrition and Health of IHM Anhui Agricultural University  Hefei China"},{"name":"Anhui Province Key Laboratory of Pollution Damage and Biological Control for Huaihe River Basin Fuyang Normal University  Fuyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010yu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Joint Research Center for Food Nutrition and Health of IHM Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010xin","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Joint Research Center for Food Nutrition and Health of IHM Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010xiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Joint Research Center for Food Nutrition and Health of IHM Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai\u2010ping","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Joint Research Center for Food Nutrition and Health of IHM Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Kong","sequence":"additional","affiliation":[{"name":"Shandong Pengbo Biotechnology Co., Ltd.  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Pengbo Biotechnology Co., Ltd.  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7599-9400","authenticated-orcid":false,"given":"Ying\u2010lao","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Joint Research Center for Food Nutrition and Health of IHM Anhui Agricultural University  Hefei 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