{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T04:22:35Z","timestamp":1771129355382,"version":"3.50.1"},"reference-count":76,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1401100"],"award-info":[{"award-number":["2023YFD1401100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272565"],"award-info":[{"award-number":["32272565"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>The extensive use of herbicides has accelerated the evolution of weed resistance in paddy fields, creating an urgent need for innovative agricultural solutions. Developing herbicide\u2010resistant rice represents an important approach to managing paddy weeds and mitigating the evolution of resistant weeds. This study explores the resistance mechanisms in ethyl methanesulfonate (EMS)\u2010induced rice, focusing on target\u2010site resistance (TSR) and non\u2010target\u2010site resistance (NTSR), while examining their influence on key agronomic traits.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The rice mutant exhibited an 18.52\u2010fold resistance to quizalofop\u2010\n                      <jats:italic>p<\/jats:italic>\n                      \u2010ethyl, conferred jointly by the ACCase Trp1999Leu mutation and GST\u2010mediated detoxification. While ACCase transcript levels remain unchanged between mutant and wild\u2010type, the mutant's enzyme activity is markedly higher; quizalofop\u2010acid residues are significantly lower in the mutant after 72\u2009h exposure, evidencing metabolic resistance. After treatment with malathion and NBD\u2010Cl, the mutant\u2019s sensitivity to the herbicide increased by 16.94% and 30.1%, respectively. The mutant also exhibited cross\u2010resistance to other ACCase herbicides. Although minor agronomic differences were observed, theoretical yield remained unaffected, demonstrating that resistance was achieved without a yield penalty.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      EMS\u2010induced rice mutants exhibit resistance to quizalofop\u2010\n                      <jats:italic>p<\/jats:italic>\n                      \u2010ethyl, through a combination of target\u2010site mutations and enhanced GST\u2010mediated\u00a0metabolism. Importantly, no detrimental impacts on agronomic performance or yield were observed. These findings enhance our understanding of resistance mechanisms and offer valuable guidance for advancing resistance breeding and genetic improvement in rice cultivation. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70368","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T09:13:26Z","timestamp":1763370806000},"page":"2313-2332","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual resistance mechanisms in\n                    <scp>EMS<\/scp>\n                    \u2010induced rice:\n                    <scp>ACCase Trp1999Leu<\/scp>\n                    mutation and metabolic resistance to quizalofop\u2010\n                    <scp>\n                      <i>p<\/i>\n                    <\/scp>\n                    \u2010ethyl"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5766-7758","authenticated-orcid":false,"given":"Bin","family":"Chen","sequence":"first","affiliation":[{"name":"School of Tropical Agriculture and Forestry Hainan University  Haikou China"},{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xitie","family":"Ling","sequence":"additional","affiliation":[{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3828-4988","authenticated-orcid":false,"given":"Zhenzhen","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honghao","family":"Mao","sequence":"additional","affiliation":[{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Xiao","sequence":"additional","affiliation":[{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeyu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhongshan Biological Breeding Laboratory Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baolong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Tropical Agriculture and Forestry Hainan University  Haikou China"},{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8451-6084","authenticated-orcid":false,"given":"Xia","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Germplasm Resources and Biotechnology Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"247","article-title":"Effect of pyraclonil on weed control in bi\u2010cropped rice fields and subsequent crops","volume":"46","author":"Zhang Y","year":"2020","journal-title":"Plant Prot"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2021.05.007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy12112724"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy14010126"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e26527"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8373"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.106240"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.86.4.983"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy12071635"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/wet.2024.3"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4675"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.105826"},{"key":"e_1_2_8_15_1","article-title":"Breeding herbicide\u2010resistant rice (Oryza sativa) using CRISPR\/Cas gene editing and other technologies","volume":"5","author":"Luo Q","year":"2024","journal-title":"Plant Commun"},{"key":"e_1_2_8_16_1","unstructured":"HeapI The International Herbicide\u2010Resistant Weed Databasehttp:\/\/www.weedscience.org\/Home.aspx(accessed August 7 2024)."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6734"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-021-00942-w"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15552"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105691"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6040"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.625138"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2019.02.010"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.636652"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2012.08.008"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14491"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2019.05.019"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8331"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c01889"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.993"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31023-x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(02)00007-3"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1017\/wsc.2021.49"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0393-7"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/horticulturae10060553"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(15)61219-9"},{"key":"e_1_2_8_37_1","first-page":"45","article-title":"EMS induced morphometric biomass and phytochemical variations in morus species (genotype RFS135)","volume":"3","author":"Kumar H","year":"2013","journal-title":"AJEA"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top108504"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1614\/WS-D-13-00063.1"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv248"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-04280-x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2020.03.037"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010528"},{"key":"e_1_2_8_46_1","unstructured":"Standardization Administration of China Cereals and feedstuffs for import and export\u2010Method for the determination of germinative energy and germination (SN\/T 0800.14\u20131999)(1999)."},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fcr.2016.12.003"},{"key":"e_1_2_8_48_1","first-page":"242","article-title":"Approaches to modeling of biological experimental data with Graphpad prism software","volume":"13","author":"Mavrevski R","year":"2018","journal-title":"WSEAS Trans Syst Control"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-019-0976-1"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/wbm.12262"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2019.05.004"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3180.2012.00919.x"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.09.035"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14101"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0110591"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21259"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep19104"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42994-022-00086-1"},{"key":"e_1_2_8_59_1","article-title":"Ala179Val mutation in rice acetolactate synthase confers broad\u2010spectrum resistance to ALS inhibitor herbicides","volume":"25","author":"An C","year":"2024","journal-title":"J Plant Genet Res"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants8090341"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1017\/wsc.2017.10"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105530"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2024.120120"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2022.105127"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2022.128225"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.134078"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.2133"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2011.12.018"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1331725"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3753"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10112350"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-023-01482-1"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.17.00959"},{"key":"e_1_2_8_74_1","first-page":"209","article-title":"The impact of the plastid ACCase Trp\u20101999\u2010Leu mutation on the growth fitness of Beckmannia syzigachne in wheat fields","volume":"33","author":"Bai S","year":"2024","journal-title":"Acta Pratac Sin"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13352"},{"key":"e_1_2_8_76_1","first-page":"72","article-title":"Development and identification of rice seed quality with ACCase inhibitor herbicides","volume":"2","author":"Wu DD","year":"2024","journal-title":"China Seed Ind"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01078"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.70368","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.70368","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.70368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T03:25:37Z","timestamp":1771125937000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.70368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,17]]},"references-count":76,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/ps.70368"],"URL":"https:\/\/doi.org\/10.1002\/ps.70368","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,17]]},"assertion":[{"value":"2025-02-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}