{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T04:33:31Z","timestamp":1774067611892,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T00:00:00Z","timestamp":1656720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000943","name":"Commonwealth Scientific and Industrial Research Organisation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000943","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012752","name":"Elanco Animal Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012752","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The sheep blowfly, <jats:italic>Lucila cuprina<\/jats:italic>, is a myiasis\u2010causing parasite responsible for significant production losses and welfare issues for the Australian sheep industry. Control relies largely on the use of insecticides. The pyrimidine compound, dicyclanil, is the predominant control chemical, although other insecticides also are used, including imidacloprid, ivermectin, cyromazine and spinosad. We investigated <jats:italic>in vitro<\/jats:italic> resistance patterns and mechanisms in field\u2010collected blowfly strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Walgett 2019 strain showed significant levels of resistance to both dicyclanil and imidacloprid, with resistance factors at the IC<jats:sub>50<\/jats:sub> of 26\u2010 and 17\u2010fold, respectively, in <jats:italic>in vitro<\/jats:italic> bioassays. Co\u2010treatment with the cytochrome P450 inhibitor, aminobenzotriazole, resulted in significant levels of synergism for dicyclanil and imidacloprid (synergism ratios of 7.2\u2010 and 6.1\u2010fold, respectively), implicating cytochrome P450 in resistance to both insecticides. <jats:italic>Cyp12d1<\/jats:italic> transcription levels were increased up to 40\u2010fold throughout the larval life stages in the resistant strain compared to a reference susceptible strain, whereas transcription levels of some other <jats:italic>cyp<\/jats:italic> genes (<jats:italic>6g1<\/jats:italic>, <jats:italic>4d1<\/jats:italic>, <jats:italic>28d1<\/jats:italic>) did not differ between the strains. Similar resistance levels also were observed in flies collected from the same property in two subsequent years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study indicates that <jats:italic>in vitro<\/jats:italic> resistance to both dicyclanil and imidacloprid in this field\u2010collected blowfly strain is likely mediated by cytochrome P450, with Cyp12d1 implicated as the enzyme responsible; however, it remains possible that another P450 also may be involved. A common resistance mechanism for the two drugs has important implications for drug rotation strategies designed to prolong the useful life of flystrike control chemicals. \u00a9 2022 The Authors. <jats:italic>Pest Management Science<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7037","type":"journal-article","created":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T02:17:59Z","timestamp":1655000279000},"page":"4195-4206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Resistance to dicyclanil and imidacloprid in the sheep blowfly, <i>Lucilia cuprina<\/i>, in Australia"],"prefix":"10.1002","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8224-7634","authenticated-orcid":false,"given":"Andrew C","family":"Kotze","sequence":"first","affiliation":[{"name":"CSIRO Agriculture and Food  St. Lucia, Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil H","family":"Bagnall","sequence":"additional","affiliation":[{"name":"CSIRO Agriculture and Food  St. Lucia, Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela P","family":"Ruffell","sequence":"additional","affiliation":[{"name":"CSIRO Agriculture and Food  St. Lucia, Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah D","family":"George","sequence":"additional","affiliation":[{"name":"Elanco Australasia Pty Ltd  Kemps Creek New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas M","family":"Rolls","sequence":"additional","affiliation":[{"name":"Elanco Australasia Pty Ltd.  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