{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T19:28:58Z","timestamp":1783970938765,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2017YFD020030106"],"award-info":[{"award-number":["2017YFD020030106"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>\u03c9\u2010hexatoxin\u2010Hvn1b is an insecticidal toxin produced by the Tasmanian funnel\u2010web spider (<jats:italic>Hadronyche venenata<\/jats:italic>), that can be exploited for development of novel bioinsecticides. Due to its larger size and low membrane permeability, this toxin usually has a slower mode of action compared to conventional small molecule insecticides. Nanoscale materials have unique optical, electrical, mechanical and biological properties, and show great application prospects for pesticide delivery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The physical and chemical properties of nanocapsules were characterized using transmission electron microscopy, laser particle size analysis, Fourier transform infrared spectroscopy, contact angle testing and with a fluorescence spectrophotometer. The results indicated that the nanocapsules were spherical, with an average particle size of 197.70\u2009nm, the encapsulation efficiency rate was 75.82% and the Zeta potential was \u221232.90\u2009mV. Penetration experiments showed that the nanocapsules could promote protein passage through the intestinal tract of <jats:italic>Spodoptera litura<\/jats:italic> and reach the body fluid. Then we expressed \u03c9\u2010hexatoxin\u2010Hvn1b by prokaryotic expression. Bioassay results showed that the oral toxicity of \u03c9\u2010hexatoxin\u2010Hvn1b nanocapsules to <jats:italic>S.\u00a0litura<\/jats:italic> was higher than that of the \u03c9\u2010hexatoxin\u2010Hvn1b.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this paper, we reported a construction method of spider venom peptide nanocapsules based on polylactic\u2010co\u2010glycolic acid by multiple emulsion for delivery of protein to improve the insecticidal effect and oral activity of \u03c9\u2010hexatoxin\u2010Hv1a. \u00a9 2022 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.7045","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T06:14:42Z","timestamp":1655532882000},"page":"4261-4267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Preparation and performance characteristics of spider venom peptide nanocapsules"],"prefix":"10.1002","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6554-731X","authenticated-orcid":false,"given":"Zheng","family":"Wei","sequence":"first","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhu","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinya","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zitong","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenchen","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunyan","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuqian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"He","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wu","sequence":"additional","affiliation":[{"name":"The National Center for Nanoscience and Technology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangmao","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Plant Protection Southwest University  Chongqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"167","article-title":"Pesticides, food safety and integrated Pest management","volume":"3","author":"Dharam PA","year":"2014","journal-title":"J Integr Pest Manag"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2017.12.017"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2012.06.012"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2006.07.006"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-011-0404-z"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2013.11.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins4030191"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syz064"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins4030191"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-2016-x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41048-018-0067-x"},{"key":"e_1_2_9_13_1","first-page":"90","article-title":"Evaluation of chemical strategies for improving the stability and Oral toxicity of insecticidal peptides","volume":"6","author":"Herzig V","year":"2018","journal-title":"Biomed"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.01.018"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120811-153650"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2018.11.298"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800197-4.00008-7"},{"key":"e_1_2_9_18_1","first-page":"547","article-title":"Molecular evolution, functional variation, and proposed nomenclature of the gene family that includes sphingomyelinase D in Sicariid spider venoms","volume":"26","author":"Greta JB","year":"2009","journal-title":"Mol Biol Evol"},{"key":"e_1_2_9_19_1","first-page":"90","article-title":"Evaluation of chemical strategies for improving the stability and Oral toxicity of insecticidal peptides","volume":"6","author":"Volker H","year":"2018","journal-title":"Biomed"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039389"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.145832"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/08820139.2019.1695134"},{"key":"e_1_2_9_23_1","first-page":"200","article-title":"Formulation of lipid core nanocapsules","volume":"378","author":"Venturini CG","year":"2010","journal-title":"Colloid Surface A"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120478"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16948-4"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03399-0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/v7010422"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b10967"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.9b02579"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6RA07468E"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.7045","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.7045","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.7045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:28:33Z","timestamp":1761053313000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.7045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,11]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/ps.7045"],"URL":"https:\/\/doi.org\/10.1002\/ps.7045","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,11]]},"assertion":[{"value":"2021-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}