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Members of the genus\n                      <jats:italic>Streptomyces<\/jats:italic>\n                      combine broad antagonistic activity with plant immune priming and growth promotion, yet their ecological roles and field reliability remain insufficiently understood. We investigated the soil actinobacterium\n                      <jats:italic>Streptomyces murinus<\/jats:italic>\n                      JS029 in the\n                      <jats:italic>Brassica rapa\u2013Sclerotinia minor<\/jats:italic>\n                      pathosystem to elucidate its mechanisms of pathogen suppression, plant immune activation and rhizosphere microbiome modulation, and to develop a field\u2010deployable formulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Streptomyces murinus<\/jats:italic>\n                      JS029 secreted chitinase, cellulase and protease and produced the polyene macrolides pentamycin and filipin I, which are known to disrupt fungal cell walls\u00a0and plasma membranes. Plants exposed to\n                      <jats:italic>Streptomyces murinus<\/jats:italic>\n                      JS029 exhibited strong activation of salicylic acid, jasmonic acid and ethylene signalling pathways and were associated with enhanced biomass and strong protection against\n                      <jats:italic>Sclerotinia minor<\/jats:italic>\n                      . A barley\u2010based solid formulation ensured reproducible field efficacy, reducing disease by approximately 70\u201380%. Rhizosphere sequencing revealed increased fungal richness and distinct bacterial\u2013fungal compositional shifts, indicating functional reassembly of the soil microbiome. Genome analysis and ultraviolet (UV) mutagenesis linked antifungal activity to a polyene biosynthetic gene cluster.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Streptomyces murinus<\/jats:italic>\n                      JS029 integrates direct antagonism, immune priming and microbiome restructuring to support disease\u2010suppressive soils. These findings provide mechanistic insights and a practical framework for deploying multifunctional\n                      <jats:italic>Streptomyces<\/jats:italic>\n                      bioinoculants as sustainable alternatives to chemical fungicides in integrated pest management systems. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70544","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T06:43:18Z","timestamp":1767940998000},"page":"4304-4318","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A multifunctional\n                    <i>Streptomyces murinus<\/i>\n                    bioinoculant integrates immune priming and microbiome restructuring for sustainable control of\n                    <i>Sclerotinia minor<\/i>\n                    in\n                    <i>Brassica rapa<\/i>"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2032-0964","authenticated-orcid":false,"given":"Miju","family":"Jo","sequence":"first","affiliation":[{"name":"Department of Plant Medicine Sunchon National University  Suncheon Republic of Korea"},{"name":"Interdisciplinary Program in IT\u2010Bio Convergence System (BK21 plus) Sunchon National University  Suncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1164-0369","authenticated-orcid":false,"given":"Sunmin","family":"An","sequence":"additional","affiliation":[{"name":"Department of Plant Medicine Sunchon National University  Suncheon Republic of Korea"},{"name":"Interdisciplinary Program in IT\u2010Bio Convergence System (BK21 plus) Sunchon National University  Suncheon Republic of 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