{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:50:37Z","timestamp":1790621437596,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Pesticides are an irreplaceable means of guaranteeing food security. The development of new types of highly efficient, low\u2010toxicity and low\u2010residue pesticides, together with the promotion of pesticide reduction and green developments are important directions of research. Based on this, novel cyanoacrylate derivatives bearing a phosphate or fluoro fragment were synthesized as potential myosin\u20105 inhibitors using an efficient screening method, namely a stepwise optimization strategy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      A single crystal of compound\n                      <jats:bold>D11<\/jats:bold>\n                      as a structural representative was scanned using an X\u2010ray diffractometer to further determine its structure. The optimized compound\n                      <jats:bold>D6<\/jats:bold>\n                      had an excellent half\u2010maximal effective concentration (EC\n                      <jats:sub>50<\/jats:sub>\n                      ; 0.40\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      )\n                      <jats:italic>in vitro<\/jats:italic>\n                      against\n                      <jats:italic>Fusarium graminearum<\/jats:italic>\n                      that was very close to that of the fungicide phenamacril (0.17\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ).\n                      <jats:italic>In vivo<\/jats:italic>\n                      tests on wheat leaves revealed that compound\n                      <jats:bold>D6<\/jats:bold>\n                      had outstanding antifungal efficacy. The effect of treatment with optimized compound\n                      <jats:bold>D6<\/jats:bold>\n                      (25\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) on mycelium morphology was demonstrated in a scanning electron microscope experiment, and was consistent with the cell membrane permeability results. Comparisons of the molecular structure and density functional theory calculations revealed that compound\n                      <jats:bold>D6<\/jats:bold>\n                      had similar thermodynamic and geometrical structure parameters to phenamacril. Furthermore, molecular docking verified that the binding mode between compound\n                      <jats:bold>D6<\/jats:bold>\n                      and myosin\u20105 was consistent with phenamacril. The complexes 6UI4\u2013\n                      <jats:bold>D6<\/jats:bold>\n                      and 6UI4\u2013phenamacril had similar trajectories and close values for total energy and hydrogen bonding.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This research constructing novel cyanoacrylate derivatives bearing a phosphate or fluoro fragment as potential myosin\u20105 inhibitors provides a valuable antifungal lead for development of an efficient fungicide. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70584","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T09:54:07Z","timestamp":1769680447000},"page":"4688-4701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Systematic optimizations, antifungal evaluation, mechanism study and potential myosin\u20105 inhibitor: development of novel cyanoacrylate derivatives bearing phosphate or fluoro fragment"],"prefix":"10.1002","volume":"82","author":[{"given":"Feng","family":"Peng","sequence":"first","affiliation":[{"name":"College of Sciences, Nanjing Agricultural University  Nanjing China"},{"name":"Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Sciences, Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Sciences, Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aobei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Sciences, Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Sciences, Nanjing Agricultural University  Nanjing China"},{"name":"Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-0077","authenticated-orcid":false,"given":"Chunlong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Sciences, Nanjing Agricultural University  Nanjing China"},{"name":"Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingguo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Nanjing Jixing Biotechnology Development Co., Ltd  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7218"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2018.03.020"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5229"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2020.104669"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.113.S3.1030"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8031"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c03399"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8388"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1237227"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006827"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.43.113004.133839"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5454"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b05942"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2011.00783.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10947"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c03755"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-018-9891-7"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7197"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2007.04.010"},{"key":"e_1_2_8_21_1","first-page":"3312","article-title":"Synthesis crystal structure and biological activities of 2\u2010cyanoacrylates containing furan or tetrahydrofuran moieties","volume":"75","author":"Liu Y","year":"2019","journal-title":"J Agric Food Chem"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2017.12.007"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jscs.2024.101928"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02980812"},{"key":"e_1_2_8_25_1","first-page":"38","article-title":"Advances in research on organophosphorus fungicides","volume":"2","author":"Li Y","year":"1978","journal-title":"Yunnan Chem Technol"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/10426507.2014.887076"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/10426507.2012.681408"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0cs00221f"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200300833"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200301023"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c00830"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101467"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.17.123"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100785a001"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1829"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9SC04185K"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm401375q"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408440500534362"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7921"},{"key":"e_1_2_8_40_1","first-page":"29","article-title":"Current status of research, development and production of fluorinated fungicides","volume":"1","author":"Liang Z","year":"2004","journal-title":"Organo Fluorine Ind"},{"key":"e_1_2_8_41_1","first-page":"1775","article-title":"Novel short\u2010step synthesis of functionalized \u03b2\u2010phenyl\u2010\u03b2\u2010hydroxypropenoates and their cyclization to 4\u2010hydroxycoumarins via the N\u2010hydroxybenzotriazole methodology","volume":"11","author":"Athanasellis G","year":"2004","journal-title":"Synthesis"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1341067"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c00920"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c04933"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8708"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c07880"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c00126"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8617"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8323"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08248"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8448"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.70584","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.70584","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.70584","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T04:49:53Z","timestamp":1776055793000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.70584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,29]]},"references-count":50,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/ps.70584"],"URL":"https:\/\/doi.org\/10.1002\/ps.70584","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,29]]},"assertion":[{"value":"2025-09-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}