{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:35:50Z","timestamp":1791725750636,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Broad bean wilt virus 2 (BBWV2) is a major viral pathogen causing significant economic losses in vegetable production. Existing detection methods often lack the speed, sensitivity, or simplicity required for effective on\u2010site field diagnosis. This study aimed to identify a novel BBWV2 isolate and develop a rapid, equipment\u2010free visual detection method suitable for field applications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      A novel BBWV2 isolate (BBWV2\u2010GZCa) was identified and characterized from pepper in Guizhou Province, China. We developed a one\u2010step visual detection assay by integrating reverse transcription\u2010recombinase polymerase amplification (RT\u2010RPA) with a CRISPR\/Cas12a system, enabling readout\n                      <jats:italic>via<\/jats:italic>\n                      fluorescence or lateral flow dipstick (LFD). The assay demonstrated a detection limit of 7.5 copies\/\u03bcL, which is 10\n                      <jats:sup>5<\/jats:sup>\n                      times more sensitive than conventional RT\u2010PCR. It showed no cross\u2010reactivity with other common pepper viruses and achieved 100% accuracy when validated using 20 field\u2010collected samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>The entire detection process can be completed within one hour without specialized equipment, requiring only visual interpretation. This RT\u2010RPA\u2010CRISPR\/Cas12a\u2010LFD method provides a rapid, highly sensitive, specific, and user\u2010friendly platform for on\u2010site detection of BBWV2, offering a practical tool for early diagnosis and disease management in agricultural settings. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70635","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T06:39:20Z","timestamp":1770878360000},"page":"5255-5265","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of a novel\n                    <scp>BBWV2<\/scp>\n                    isolate and a sensitive and rapid\n                    <scp>RT<\/scp>\n                    \u2010\n                    <scp>RPA<\/scp>\n                    \u2010\n                    <scp>CRISPR<\/scp>\n                    \/\n                    <scp>C<\/scp>\n                    as12a\u2010\n                    <scp>LFD<\/scp>\n                    detection method development"],"prefix":"10.1002","volume":"82","author":[{"given":"Ke","family":"Han","sequence":"first","affiliation":[{"name":"Institute of Crop Protection, College of Agriculture, Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Crop Protection, College of Agriculture, Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Crop Protection, College of Agriculture, Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Crop Protection, College of Agriculture, Guizhou University  Guiyang 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