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Although herbivory remodels plant methylomes, the links between DNA methylation, resistance traits, and phytohormone signaling remain unresolved.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      We induced genome\u2010wide hypomethylation in potato plants,\n                      <jats:italic>Solanum tuberosum<\/jats:italic>\n                      L., using the DNA methyltransferase inhibitor 5\u2010azacytidine (5\u2010azaC), and assessed their resistance to the potato tuber moth (PTM),\n                      <jats:italic>Phthorimaea operculella<\/jats:italic>\n                      Zeller. Foliar application of 100\u2009\u03bc\n                      <jats:sc>m<\/jats:sc>\n                      5\u2010azaC significantly reduced global DNA methylation in leaves and compromised resistance to\n                      <jats:italic>Phthorimaea operculella<\/jats:italic>\n                      . Larvae and adults displayed stronger feeding and oviposition preferences, respectively, for 5\u2010azaC\u2010treated plants and larvae gained 25.9% more weight on treated than on control plants. These dual defects were associated with marked decreases in trypsin proteinase inhibitor (TPI) accumulation and emission of the anti\u2010herbivore volatiles such as\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010caryophyllene. Moreover, hypomethylated plants had substantially lower levels of jasmonic acid (JA) and transcripts of JA biosynthetic genes. Exogenous methyl jasmonate (MeJA) restored resistance to\n                      <jats:italic>Phthorimaea operculella<\/jats:italic>\n                      by reinstating TPI accumulation and\n                      <jats:italic>\u03b2\u2010<\/jats:italic>\n                      caryophyllene emission, whereas silencing of allene oxide cyclase gene (\n                      <jats:italic>StAOC<\/jats:italic>\n                      ) caused opposite phenotype. Moreover, JA modulated genome\u2010wide DNA methylation during herbivory and\n                      <jats:italic>Phthorimaea operculella<\/jats:italic>\n                      herbivory reduced promoter methylation of JA\u2010related genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Disrupting DNA methylation homeostasis compromises potato defenses and is associated with reduced JA\u2010regulated defense outputs. Our findings reveal dynamic interplay between DNA methylation and JA\u2010mediated herbivore defense in potato. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.70636","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T06:31:09Z","timestamp":1771223469000},"page":"5303-5316","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome\u2010wide\n                    <scp>DNA<\/scp>\n                    methylation homeostasis influences jasmonate signaling and potato defenses against the potato tuber moth\n                    <scp>\n                      <i>Phthorimaea operculella<\/i>\n                    <\/scp>"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3155-6735","authenticated-orcid":false,"given":"Yixin","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory 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